摘要
目的观察大鼠脑出血(ICH)后基质金属蛋白酶-9(MMP-9)蛋白动态表达及其与血肿周围脑组织含水量的关系。方法将雌雄各半的Wistar大鼠170只随机分为3组:正常对照组、假手术组、ICH组。每组均观察手术后6h、12h、24h、48h、72h、120h、7d、15d八个时间点,测定血肿周围脑组织含水量、MMP-9蛋白表达。结果ICH后MMP-9蛋白表达在24h^48h达高峰,与对照组相比,差异有统计学意义,与血肿周围脑组织含水量呈正相关(r=0.949,P<0.01)。结论大鼠ICH后MMP-9蛋白的表达是ICH后脑水肿形成主要因素。
Objective To observe the relationship between the changes of contents of matrix metalloproteinase-9 （MMP-9） protein and water contents in perihematoma in rats with intracerebral hemorrhage. Methods 170 Wistar rats of half male and half female were assigned randomly to three groups： normal control group, sham operation group, and ICH group. The water contents in perihematoma and the expression of MMP-9 protein were measured respectively at 8 time points of 6h, 12h, 24h, 48h, 72h, 120h, 7d, and 15d after operation in every group. Results The contents of MMP-9 protein at 24h -48h were higher in comparison with other time points and the control group（P〈0. 01）. The changes of the contents of MMP-9 protein were positively correlated with water contents in perihematoma （r=0. 949, t9 d0.01） after ICH. Conclusion The expression of MMP-9 protein is an important factor relating to brain edema after ICH.
出处
《西部医学》
2007年第5期767-769,共3页
Medical Journal of West China
基金
四川省卫生厅重点建设基金项目(No.050082)
关键词
基质金属蛋白酶-9
脑出血
脑水肿
Matrix metalloproteinase-9
Intracerebral hemorrhage Brain edema
