摘要
目的:建立人胃癌顺铂耐药细胞系方法:采用逐步递增顺铂浓度,间歇作用体外诱导法建立人胃癌顺铂耐药细胞系SGC7901/ DDP,每月作相关检测;MTT法测定药物敏感性:光镜、电镜、MTT法计数活细胞、流式细胞仪等方法观察其生物学特征的改变.结果:历时4 mo建成人胃癌顺铂耐药细胞系SGC7901/DD P,1,2,3,4mo的耐药指数分别为2.13,5.32,12.60,12.93,并且与5-氟尿嘧啶、丝裂霉素等多种抗癌药有不同程度的交叉耐药性:SGC7901/DDP的细胞形态逐渐发生改变;体外群体倍增时间较亲代细胞逐渐延长:细胞周期分析发现其S期与G_2/M期细胞逐渐减少,G_0/G_1期细胞逐渐增多.结论:SGC7901/DDP细胞具有耐药表型特征,耐药性能稳定,增殖能力逐渐下降.
AIM： To establish a cisplatin-induced human gastric cancer drug-resistant cell line and study changes in its resistance.
METHODS： A resistant gastric cancer cell line （SGC7901/DDP） was established by gradually increasing the dose of cisplatin with intermittent, monthly administration. Drug sensitivity was detected by MTT assay. Changes in biological characteristics were determined using light microscopy, electron microscopy, cell counting by MTT assay, and flow cytometry.
RESULTS： The SGC7901/DDP cell line was developed after 4 mo, with stable resistance to cisplatin. The resistance index for each month was 2.13, 5.32, 12.60 and 12.93, respectively.SGC7901/DDP cells exhibited cross-resistance to many other chemotherapeutic agents （e.g., 5-fuorouracil and mitomycin C）. The morphology of SGC7901/DDP cells gradually changed; doubling time was gradually prolonged, and the cell numbers in S- and G2/M-phases gradually decreased, while that in G0/G1 phase gradually increased, compared with parent cells.
CONCLUSION： The SGC7901/DDP cell line showed a typical and stable resistant phenotype, multiplication of the SGC7901/DDP cell line gradually decreased, and MTT assay and electron microscopy determined the resistance of the SGC7901/DDP cell line.
出处
《世界华人消化杂志》
CAS
北大核心
2007年第18期2009-2013,共5页
World Chinese Journal of Digestology
作者简介
通讯作者：潘洪明,副教授,硕士生导师,主要研究中药多糖抗肿瘤机制.161042,齐齐哈尔市富拉尔基区,齐齐哈尔医学院生物化学教研室.qyphm@sohu.com 电话：0452-6731101
