摘要
目的观察针刺对宫内感染致脑损伤仔鼠脑组织胶质纤维酸性蛋白(GFAP)表达影响。方法对孕17、18d脂多糖(LPS)组大鼠腹腔注射LPS,建立宫内感染致脑损伤仔鼠动物模型,以腹腔注射9g/L盐水为对照组。LPS组仔鼠随机分为针刺组与模型组,针刺组自7～21日龄予针刺干预。3组分别于生后21d取幼鼠脑组织应用免疫组织化学方法检测脑白质GFAP表达。结果针刺组GFAP免疫阳性细胞数量多,染色深;对照组GFAP免疫阳性细胞数量少,染色浅;模型组介于二者间。结论GFAP表达增加可能是针刺对宫内感染致脑损伤仔鼠的治疗机制之一。
Objective To observe the effect of acupuncture on cerebral glial fibrillary acidic protein（GFAP） expression of brain injury caused by intrauterine iofeotion in neonatal rats. Methods Forty - thee Wistar pregnant rats were divided into 2 groups：lipopolysaccharide （LPS） （n = 35 ）and normal saline（ n = 8）. Pregnant rats were consecutively injected intraperitoneally with LPS（450 μg/kg）or saline on gestation 17 d and 18 d. LPS group were randomly divided into acupuncture group and model group. Acupuncture group were given acupunctured from 7 d to 21 d. GFAP expression was assayed with immunohistochemical technique. Results The number of GFAP immunoreaction（IR） strongly-positive neurons in the cerebral white matter was more in acupuncture group,less in model group and only very weakly 1R- positive neurons were found in normal saline group. Condusion Acupuncture can up - regulate the expression of GFAP in the cerebral white matter, which may contribute to the effect of acupuncture in improving brain injury caused by intrauterine infection.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2007年第15期1168-1169,1171,共3页
Journal of Applied Clinical Pediatrics
基金
黑龙江省研究生创新科研项目资助(YJSCX2007-0099HLJ)
关键词
宫内感染
脑白质损伤
针刺
胶质纤维酸性蛋白
intrauterine infection
damage of white matter
acupuncture
glial fibrillary acidic protein
作者简介
迟瑛娇，女，硕士生，研究方向为脑损伤。
