摘要
作者收集了中国食管癌三个高发区（郑州、大原、汕头）和一个低发区（广州）的食管鳞癌手术切除标本，福尔马林固定，石蜡包埋，采用CM－1和Do－7作一抗，用免疫组化LSAB法对其进行检测，在四个不同地区p53蛋白积聚率无差异（P＞0．05），阳性率为62．8％（Do－7）。因此，食管鳞癌中p53蛋白积聚是一常见现象，在不同地区，p53的改变在该肿瘤形成过程中的作用是类同的。作者还发现PCNA（Proliferatingcellnuclearantigen）的表达与p53蛋白积聚的分布规律一致，两者阳性等级密切相关（P＜0．01），说明有p53蛋白积聚的肿瘤细胞是处于细胞增殖周期中，可能与肿瘤的预后有一定的关系。
The authors studied 188 formalin-fixed, paraffin-embedded esophageal squamous cell carcinoma tissues taken from three high-incidence (Zhengzhou,Taiyuan and Shantou) and one low-incidence areas (Guangzhou)with the intention of identifying p53 protein accumulation immunohistochemically by use of CM-1 and Do-7 primary antibodies and LSAB kit. The p53 protein accumulation rate observed was 62. 8%. There was no significant difference between the accumulation rates in these four districts. Therefore,the p53 protein accumulation to be involved in the carcinogenesis is irrespective of the patients' residency in high or low incidence area. Furthermore,most of the p53 protein accumulated cancer cells are PCNA positive, a fact indicates that these two proteins are correlated with each other.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
1997年第6期405-407,共3页
Chinese Journal of Clinical Oncology
基金
美国中华医学会资助
