摘要
目的探讨结直肠癌中survivin、caspase-3和p21WAF1的蛋白表达与临床病理参数的联系以及survivin与caspase-3和p21WAF1蛋白表达之间的关系。方法采用免疫组织化学SP方法检测15例正常结直肠粘膜和62例结直肠腺癌标本中survivin、caspase-3和p21WAF1的蛋白表达。结果结直肠腺癌与正常结直肠粘膜比较,survivin、caspase-3和p21WAF1的蛋白表达差异均有显著性(P<0.05)。survivin和caspase-3的蛋白表达与淋巴结转移无明显相关(P>0.05);而与分化程度均显著相关(P<0.05)。survivin蛋白与Dukes分期相关(P<0.05);但caspase-3的蛋白表达与Dukes分期无关(P>0.05)。p21WAF1蛋白表达与分化程度、淋巴结转移和Dukes分期均显著相关(P<0.05)。survivin蛋白分别与caspase-3、p21WAF1蛋白表达之间均呈显著负相关(P<0.01)。结论survivin、caspase-3和p21WAF1蛋白在结直肠癌的发生和进展中都起着重要的作用。p21WAF1基因与结直肠癌的恶性进展显著相关,此结论鲜见报道。
Objective To investigate the expression of survivin, caspase-3 and p21^WAF1 proteins in colorectal carcinoma and their relationship between their expression and clinical pathology parameter, and correlation between expression of survivin protein and caspase-3, p21^WAF1 protein expression respectively. Methods SP immunohistochemical method was used to detect expressions of survivin, caspase-3 and p21^WAF1 proteins in 15 normal colorectal mucosa and 62 colorectal carcinoma. Results Colorectal carcinoma compared with normal colorectal mucosa, there was significant differences of survivin, caspase-3 and p21^WAF1 protein expression （P〈0. 05）. No correlation was observed between survivin, caspase-3 protein expression and lymph node metastasis （P〈0. 05）, but survivin, caspase-3 protein expression were both correlated with cell differentiation degree （P〈0. 05）. Positive rates of survivin protein was 44. 44% （16/ 36） in A and B of Dukes phase, which lower than positive rates （73.08%） of survivin protein in C and D of Dukes phase（P〈0. 05）. No correlation was observed between caspase-3 protein expression and Dukes phase （P〉0. 05）. There was significant differences between p21^WAF1 protein expression and cell differentiation degree, lymph node metastasis, Dukes phase （P〈0. 05）. A highly negative correlation was present between survivin and caspase-3, p21^WAF1 protein expression respectively（P〈0. 01）. Conclusion survivin, caspase-3 and p21^WAF1 genes may be an important role to formation and progression of colorectal carcinoma. It is rarely reported on relationship between p21^WAF1gene and the malignant progression of colorectal carcinoma.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2007年第8期596-599,644,共5页
Cancer Research on Prevention and Treatment
作者简介
梁素美（1962-），女，学士，主治医师，主要从事血液病和肿瘤化疗的研究
