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DHPLC检测奶牛乳铁蛋白基因点突变方法的研究

Study on the DHPLC Method of Screening Point Mutation of Bovine Lactoferrin Gene
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摘要 乳房炎是奶牛生产中最普遍、最复杂也是花费最多的疾病,与乳铁蛋白存在一定的相关性。本实验以DHPLC取代SSCP检测bLF基因点突变。结果表明,DHPLC对点突变的检出率达到97%,优于SSCP等方法。 Mastitis is the most frequent, complex and cosily disease in dairy cattle and has a close relationship with the lactoferrin. In this present study, DHPLC is used to screen the point mutation instead of SSCP and the 97% of the accuracy indicates DHPLC is a good method compared with SSCP, et al.
出处 《安徽科技学院学报》 2007年第4期1-4,共4页 Journal of Anhui Science and Technology University
基金 国家"十五"科技攻关重大专项(No:2002BA518A12) 安徽省教育厅自然科学研究项目(No:2006KJ201B)
关键词 乳铁蛋白基因 点突变 DHPLC Lactoferrin Point mutation DHPLC
作者简介 郭亮(1962-),男,安徽省无为县人,硕士,副教授,主要从事畜牧有机生产教学与研究。 通讯作者Correspondence author,Tel:025-84395045
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参考文献9

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