摘要
为了进一步研究蒙古黄芪(Astragalus mongholicusBunge)中的蛋白质及其功能,通过金属离子螯合层析、离子交换层析和凝胶过滤得到一种新蛋白(AmPR-10),并对其部分性质进行了研究。AmPR-10在SDS-PAGE上的分子质量为17.2 ku,凝胶过滤层析测得分子质量为32.6 ku,说明其为同源二聚体。糖蛋白染色显示为糖蛋白,总糖含量为13.7%,离子交换色谱法分析其中含有73%阿拉伯糖、15%葡萄糖、微量的果糖和一种未知糖;N-端序列分析结果显示其与病程相关蛋白家族PR-10中黄羽扇豆L1PR-10.1C同源性高达80%。同时,AmPR-10具有核糖核酸酶活性,纯AmPR-10的核糖核酸酶比活性为74.11 U/mg,这点与一些PR-10蛋白类似。
A novel pathogenesis-related class-10 protein （AmPR-10） from Astragalus mongholicus was purified and characterized. The purified protein appeared as a single band with a molecular mass of 17.2 ku on SDS-PAGE and was eluted from a Superdex-75 column with an apparent molecular weight of 32.6 ku. AmPR-10 was a glycoprotein with a neutral carbohydrate content of 13.7%. The carbohydrate was mainly composed of 73.0 % arabinose and 15.0% glucose. N-terminal amino-acid sequence of AmPR-10 was significantly homology to pathogenesis-related proteins from the PR-10 family. The sequence had 80% identity with L1PR10. 1C, a PR-10 protein from Lupinus luteus. AmPR-10had ribonuclease activity of 74.11 U/mg protein, as some other PR-10 proteins.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2007年第4期15-18,共4页
Journal of China Agricultural University
基金
北京市自然科学基金资助项目(2063029)
关键词
蒙古黄芪
病程相关蛋白PR-10
纯化
核糖核酸酶活性
Astragalus mongholicus Bunge
pathogenesis-related class10 proteins
purification
ribonuclease activity
作者简介
齐笑玮，硕士研究生；
闫巧娟，博士，副教授，通讯作者，主要从事生物质资源利用研究，E-mail：yanqj@cau.edu.cn
