摘要
昆虫细胞色素P 450与昆虫抗药性关系密切,本研究以烟实夜蛾5龄幼虫中肠的总RNA为模板,设计并合成简并引物,利用反转录-多聚酶链式反应(RT-PCR)扩增出了烟实夜蛾细胞色素P 450基因的cDNA片段,将其克隆到pMD 18-T载体进行序列测定,测序得到的491 bp的片断编码163个氨基酸残基,且该片断在阅读框内.与已公布的谷实夜蛾、棉铃虫和烟芽夜蛾细胞色素P 450氨基酸序列进行比较,其一致性分别为98.7%,98.7%和92.1%.
Cytochrome P 450 is closely related with the insecticide resistance of insect, cDNA fragment of cytochrome P 450 gene in the fifth instar larva of oriental tobacco budworm was amplified by RTPCR with total RNA as template and using a degenerate primer. The target fragment was further sequenced after being cloned into pMD 18-T vector plasmid. The sequenced result showed that 491 bp nucleotide sequence that encodes 163 amino acid residues was in the encoding frame. Compared with the known cytochrome P 450 nucleotide sequence of Helicoverpa zea,Helicoverpa armigera, and Heliothis virescens, the identity were 98.7% ,98.7% and 92.1% , respectively.
出处
《河南农业大学学报》
CAS
CSCD
北大核心
2007年第3期284-288,共5页
Journal of Henan Agricultural University
基金
河南省杰出青年基金项目(074100510013)
关键词
烟实夜蛾
细胞色素P450
基因克隆
序列分析
Helicoverpa assulta Gueenee
cytochrome P 450
gene cloning
sequence analysis
作者简介
李卫华（1978-），男，河南西华人，硕士研究生，从事昆虫分子生态学研究；
通讯作者：郭线茹．
