摘要
目的:优选重楼总皂苷提取工艺。方法:以总皂苷得率为评价指标,采用单因素试验和正交试验优选最佳提取工艺。结果:优化后重楼总皂苷的提取工艺为:70%乙醇提取两次,第一次用10倍量提取2 h,第二次用8倍量提取1.5 h;按优选出的工艺进行提取,总皂苷得率为4.24%,重楼总苷Ⅰ、Ⅱ总提取率为93.28%。结论:验证试验结果表明,优选出的工艺稳定、合理、可行。
Objective： To optimize the extraction process of total saopnins from Paris polyphylla Smith. Methods ： The single factor test and orthogonal experiment were used to determine the optimum extraction process. Results： The optimum extraction process was obtained as follows： the plant materials were extracted with 70% ethanol twice, respectively with 10BV for 2 hours and then with 8BV the solvents for 1.5 hours. The yield of total saponins could be up to 4. 24% and the total extraction rate of Paris polyphylla Ⅰ and Paris polyphylla Ⅱ was 93.28%. Conclusion： The optimum process obtained is steady, reasonable and feasible.
出处
《中药材》
CAS
CSCD
北大核心
2007年第6期726-729,共4页
Journal of Chinese Medicinal Materials
关键词
重楼
总皂苷
重楼总苷Ⅰ
重楼总苷Ⅱ
提取工艺
Paris polyphylla Smith
Total saponins
Paris polyphylla Ⅰ
Paris polyphylla Ⅱ
Extraction process
作者简介
通讯作者：田景奎（1972-），男，博士，副教授，主要从事新药研究，E-mail：tjk@zju．edu．cn，Tel：0571-88208446。
