摘要
观察了超大剂量6MeV电子线照射后大鼠脑组织NSE、S-100蛋白的动态变化。对成熟的(Sprague-dawle,SD)大鼠用6MeV电子线进行10Gy、20Gy和30Gy全脑单次照射,应用免疫组织化学法测定大鼠脑损伤后不同时间、不同剂量脑组织中稀醇化酶(Neuro-specific enolase,NSE)、S-100蛋白的相对含量。60只大鼠随机分为对照组和实验组,用6MeV电子线对实验组大鼠进行20Gy全脑单次垂直照射,分别于照射后1d、7d、14d和30d处死大鼠,取其脑组织,用免疫组织化学法测定NSE、S-100蛋白的相对含量,并与对照组进行比较。在上述时间点,脑内海马区S-100和NSE表达有时间规律,照射后7dS-100蛋白的表达和照射后24hNSE的表达组间存在一定差别。与对照组相比,实验组大鼠脑组织中NSE表达显著下降(p<0.05),S-100表达显著升高(p<0.05)。在照射后7d,上述各指标变化的幅度为30Gy组>20Gy组>10Gy组。电离辐射可诱导脑组织中S-100蛋白的表达,同时下调NSE在神经元中的表达,脑组织中S-100和NSE表达水平的变化可作为辐射诱导的急性脑损伤的敏感指标。
The work is to study the expression of NSE and S-100 protein in SD rat brain tissue irradiated by 6MeV electron beam. The whole brain of the Sprague-Dawley rats were irradiated with single dose of 10Gy, 20Gy and 30Gy by 6MeV electron. Immunohistochemical method was used to measure the contents of S-100 and NSE in brain tissue. One-ANOVA was used for analysis. The expression of S-100 and NSE after whole brain irradiation was time-dependent. Compared with that of control group, there was an increase of expression of S-100 D7 after irradiation during the observed time （p〈0.05）. On the contrary, there was a decrease of NSE 24h after radiation, disappearing nearly at D30 （p〈0.05）. The indexes above were changed with the different dose levels at 7d after radiation. Radiation can induce the expression of S-100, as well as inhibit the expression of NSE on radiation-induced acute brain injury. The changes of the expression of S-100 and NSE on the brain can act as indexes of acute brain injury induced by radiation.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
北大核心
2007年第3期185-188,共4页
Journal of Radiation Research and Radiation Processing
基金
国家自然科学基金(30670638)资助
关键词
电离辐射
脑损伤
NSE
S-100蛋白
Radiation effects, Brain injuries, Neuro-specific enolase （NSE）, S-100 protein
作者简介
涂彧，男，1965年7月出生，2002年毕业于苏州大学放射医学系，获放射医学博士学位，副教授，硕士生导师，主要研究方向：放射卫生与防护
