摘要
目的探讨在胶质瘤的光动力疗法中应用新型光敏剂ATX-S10.Na(Ⅱ)的可能性。方法首先对5种含有不同浓度的ATX-S10.Na(Ⅱ)的鼠和人的胶质瘤细胞中应用波长为670nm的激光照射,应用MTT法测定不同的肿瘤细胞对PDT治疗的反应。接着,在SD大鼠的C6胶质瘤皮下肿瘤模型应用中进行PDT治疗,探讨ATX-S10.Na(Ⅱ)介导的光化学反应所致的抗肿瘤作用。结果体外实验表明,PDT对肿瘤细胞的毒性作用既依靠ATX-S10.Na(Ⅱ)的药物浓度又依靠激光辐照的累计能量。每个细胞系的50%抑制浓度(IC50)波动于5-15μg/ml之间。在皮下肿瘤模型中,PDT治疗导致的肿瘤内部破坏面积随着激光能量的提高而增大,尤其是当照光剂量大于75J/cm时,与对照组相比有统计学意义。结论体外和在体的实验表明,新型光敏剂ATX-S10.Na(Ⅱ)介导的光动力疗法显示出极强的抗胶质瘤作用。
Objective To investigate the anti-tumor effect of a novel photosensitizer, ATX-SIO. Na （ Ⅱ ）, in photodynamic therapy （PDT） for glioma. Methods First PDT was performed in brain tumor cell lines in vitro using ATX-SIO. Na （ Ⅱ ） and a 670-nm laser light. Next, PDT was performed in the subcutaneous C6 tumor model in SD rat in vivo after intravenous administration of l0mg/kg ATX-SIO. Na （ Ⅱ ）. Results In vitro experiment showed a potent anti-tumor effect was observed in all the cell lines, which depended upon both drug concentration and laser energy. In vivo experiment showed obvious tumor damages, which were dose-dependent on the laser energy. Conclusion The results of the present study demonstrated a potent photodynamic anti-tumor effect in PDT using ATX-SIO. Na（ Ⅱ ） in vitro or in vivo.
出处
《中华神经外科杂志》
CSCD
北大核心
2007年第6期466-468,共3页
Chinese Journal of Neurosurgery
作者简介
通讯作者：李少一．Email：lishaoyi2097@hotmail．com
