摘要
圆网蛛类(妖面蛛总科+园蛛总科)是否为单系,圆网究竟经历一次进化还是多次进化,这是多年来有争论的、悬而未决的蛛形学难题之一。本文测定了包括妖面蛛总科、园蛛总科和非圆网蛛类等类群在内的9科10种蜘蛛线粒体12S rDNA、16S rDNA及核18S rDNA、28S rDNA等4个基因片段序列,并基于4个基因序列的整合数据,分别通过邻接(NJ)法、最大简约(MP)法、最大似然(ML)法和贝叶斯法(Bayesian)分析,对园蛛总科和妖面蛛总科蜘蛛之间的分子系统关系进行了探讨。系统发生结果表明:1)园蛛总科和妖面蛛总科蜘蛛不是姊妹群,从而支持这两个类群的圆网是平行演化而非同源演化的观点;2)筛器类蜘蛛并非单系发生而为多系发生。另外,依据编码大壶状腺丝蛋白-1(MaSp1)C末端非重复氨基酸序列区段的核酸序列重建的系统发生树也证实圆网蛛类并非单系发生。
The present study examined whether orb web spiders （Deinopoidea ＋ Araneoidea） are monophyletic or polyphyleticin origin？ Mitochondrial 12S, 16S and nuclear 18S, 28S rDNA fragments of ten species of spiders were amplified and sequenced. Using neighbor-joining （N J）, maximum parsimony （MP）, maximum likelihood （ML） methods and Bayesian analyses, the phylogenetic relationship between Deinopoidea and Araneoidea was inferred from the combined DNA sequences of 12S, 16S, 18S and 28S rDNA fragments. The resulting phylogeny indicated that： 1 ） Deinopoidea and Araneoidea did not form single clade. This finding well supports that the orb-web similarities between Deinopoidea and Araneoidea are more likely the result of convergent rather than homologous evolution; 2） Cribellate spiders are polyphyletic. In addition, the reconstructed phylogenetic tree based on cDNA and DNA sequences encoding C-terminal non-repetitive region of MaSpl （Major ampullate spidroin-1 ） also showed that Deinopoidea and Araneoidea are not sister groups [Acta Zoologica Sinica 53 （3）： 489-501, 2007].
出处
《动物学报》
SCIE
CAS
CSCD
北大核心
2007年第3期489-501,共13页
ACTA ZOOLOGICA SINICA
基金
安徽省高校"生物环境与生态安全"省级重点实验室资助项目
安徽省"重要生物资源保护与利用研究"重点实验室资助项目~~
作者简介
通讯作者（Corresponding author）．E-mail：panhongchun@126．com
