摘要
采用ISTA滤纸法检测得出：20%邻烯丙基苯酚悬浮种衣剂按1∶30-1∶90药种比（质量比）包衣处理“中农8号”和“长春密刺”黄瓜品种的种子,对黄瓜种子安全,种子发芽率分别达到95.75%-97.75%和80.00%-84.25%;PDA平板上检测药剂包衣处理对黄瓜种子带菌消毒效果显示,消毒处理效果达到66.67%-100%。菌丝生长速率法和染色处理结合显微技术实验证实,邻烯丙基苯酚对危害黄瓜秧苗的土传病原菌腐霉病菌Pythium aphanidermatum和立枯丝核菌Rhizoctonia solani具有较强的抑制作用,EC50值分别为27.59和34.16μg/mL;药剂处理使供试病菌的菌丝发生中空、解体断裂等异常现象。20%邻烯丙基苯酚悬浮种衣剂1∶30-1∶90包衣处理黄瓜种子后接种供试病菌,对腐霉病菌和立枯丝核菌的防病效果分别达到73.31%-100%和85.16%-100%;随着药剂包衣处理浓度的增大,邻烯丙基苯酚防治两种病原菌侵染危害黄瓜秧苗的效果提高,等效或者优于常规药剂福美双种子处理。
Germination safety to cucumber seeds （two cultivars Zhongnong No. 8 and Changchun Mici） ,coated with 20% o-allylphenol seed coating formulation at the ratio of 1：30 - 1：90 （ratios of seed weight）was tested by ISTA＇s blotter paper method. The results showed that o-allylphenol seed treatment is safe to seed germination, the germination ratios for both cultivars were 95.75 % - 97.75 % and 80.00% -84.25% ,respectively. Based on the PDA test,the control effect of seed disinfection was up to 66. 67% - 100%. Hyphal growth rate test and microscopy demonstrated that o-allylphenol markedly inhibited the growth of two harmful soilborne pathogens, Pythium aphanidermatum and Rhizoctonia solani, with EC50 value ranging from 27.59 to 34.16 μg/mL. The o-allylphenol treatment resulted in abnormal appearance of fungal hypha, such as broken and cavity. When the cucumber seeds were treated with 20% o-allylphenol seed coating formulation as previous ratios before inoculated with P. aphanidermatum and R. solani, the disease control efficiency was 73.31% - 100% and 85.16% - 100% ,respectively. The control efficacy to both cucumber seedling diseases rose as the o-allylphenol concentration increased. The efficacy was equal or superior to that of regular dressing treatment with thiram.
出处
《农药学学报》
CAS
CSCD
2007年第2期131-136,共6页
Chinese Journal of Pesticide Science
基金
国家科技攻关项目(2004BA521B04)
深圳市科技与信息局计划项目(S040161)
教育部"新世纪优秀人才支持计划"(NCET-04-0128)资助
关键词
邻烯丙基苯酚
腐霉病菌
立枯丝核菌
黄瓜种子
包衣处理
防病效果
o-allylphenol
Pythium aphanidermatum
Rhizoctonia solani
cucumber seed
seed coating treatment
disease control effect
作者简介
曹锦（1981-），女，青海人，硕士研究生，E-mail：rabbit1123@163．com；
通讯作者（Author for correspondence）：李健强（1964-），男，湖南人，教授，从事种子病理药理学研究．联系电话：010-62734938；E-mail：lijq231@cau．edu．cn
