摘要
研究了大花蕙兰茎尖诱导原球茎、原球茎增殖及分化再生植株的条件。结果表明,茎尖在1/2MS+NAA0.2 mg/L+BA 3.0 mg/L的培养基上培养,原球茎诱导率最高;原球茎增殖的适宜培养基为1/2MS+BA 5.0mg/L+NAA 1.0mg/L+AC 0.2%;原球茎分化的适宜培养基为1/2MS+BA 2.0 mg/L+NAA 0.2 mg/L+AC 0.2%。
The optimal conditions of the induction and proliferation of protocorms and plantlet regeneration from protocorms are investigated in this experiment. The results obtained show that the highest percentage of protocorm formation from stem-tips occurs on the 1/2MS medium supplemented with NAA 0.2 mg/L and BA3.0mg/L; the proliferation of protocorms is best on the 1/ 2MS medium with the addition of BA5.0mg/L, NAA1.0mg/L and 0.2% AC; and the 1/2MS medium with BA2.0mg/L, NAA0.2mg/L and 0.2%AC is suitable for plant differentiation from proctocorms.
出处
《龙岩学院学报》
2007年第3期74-77,共4页
Journal of Longyan University
关键词
大花蕙兰
茎尖培养
原球茎
Cymbidium Hybridum
stem-tip culture
protocorm
作者简介
韩牙琴（1980-），女，福建福州人硕士生．主要研究方向：观赏植物生物技术。
通讯作者：赖钟雄（1966-），男，福建德化人，研究员，博士生导师，主要研究方向：园艺植物生物技术与遗传资源.
