摘要
采用UV-Vis光谱法,研究在pH4·2的H3PO4-KH2PO4缓冲溶液中血红蛋白(Hb)与铜(Ⅱ)-茜素红S(ARS)络合物的反应。结果表明:Hb与Cu(Ⅱ)-ARS络合物相互作用形成红色的离子缔合复合物,该复合物的最大吸收波长为537nm。在此波长下测得复合物的组成为nHb:nCu(Ⅱ):nARS=1:4:8,表观摩尔吸光系数为1·52×105L·mol-1·cm-1,Hb浓度在1·0×10-7～2·0×10-6mol·L-1范围内与吸光度呈线性关系,回归方程为A=0·0269+151675c(mol·L-1),相关系数r=0·9972。考察了溶液酸度、试剂用量、反应时间与温度、离子强度、表面活性剂等因素对Hb-Cu(Ⅱ)-ARS复合物形成的影响,并对反应机理做了初步探讨,发现Hb与Cu(Ⅱ)-ARS络合物之间主要以静电引力相结合。进一步考察了常见氨基酸及金属离子对Hb-Cu(Ⅱ)-ARS复合物形成的影响。
The reaction of hemoglobin （Hb） with copper（ Ⅱ ）-Alizarin red S （ARS） complex was studied in H3PO4-KH2PO4 buffer solution （pH 4. 2） by ultraviolet-visible spectrophotometry. The results show that the interaction of Hb and Cu（ Ⅱ ）-ARS complex produces red ionic association complex with its maximuna absorption peak at 537 nm.At the maximuna absorption, the composition of the complex was determined to be nHb：ncu（Ⅱ）：nARS=1：4：8, and the apparent molar absorptivity was 1.52×10^5 L·mol^-1·cm^-1. The concentration of Hb is linear with the absorbency in the range of 1.0×10^-7～2.0×10^-6mol·L^-1 and the regression equation was established as A=0.0269＋151675c（mol·L^-1） with the coefficient r= 0. 997 2. The effects of solution acidity, reagent amount, reaction time, temperature, ionic strength and the added surfactant were examined on the formation of the Hb-Cu（ Ⅱ ）-ARS complex. A preliminary investigation was carried out to elucidate the reaction mechanism, and it could be concluded that the Hb and Cu（ Ⅱ ）-ARS complex are combined mainly by electrostatic attraction. Further investigation was also undertaken to find out the effects of common amino acids and metallic ions on the formation of Hb-Cu（ Ⅱ ）-ARS complex.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2007年第6期1168-1171,共4页
Spectroscopy and Spectral Analysis
基金
浙江省自然科学基金项目(Y404012)
中国科学院长春应用化学研究所电分析化学国家重点实验室开放课题资助
作者简介
吴小华，女，1961年生，浙江师范大学化学与生命科学学院副教授 e-mail：sky61@zjnu．cn
