摘要
目的:了解不同方法检测甲胎蛋白(AFP)和癌胚抗原(CEA)的结果,制定质量控制计划,提高检测结果的可比性。方法:收集不同水平的AFP和CEA血清,采用室间调查的方法,统一发放样品。各实验室在规定的时间内按日常检验方法进行检测和回报结果。按检测方法分类进行结果统计。结果:按定性方法统计,AFP阴性、弱阳性、阳性样品的符合率均为100%;CEA样品的符合率依次为阴性100%,弱阳性60%,阳性85%。按定量方法统计,不同的方法检测AFP的变异系数(CV)为9.9%～73.9%,检测CEA的CV为11.5%～65.3%。结论:不同的方法检测AFP和CEA的结果可比性较差,提示应加强质量控制,提高检验质量,使检验结果间有较好的可比性。
Objective： To determine the alpha-fetoprotein （AFP） and carcinoembryonic antigen （CEA） levels by different methods and make a quality control plan to increase the comparability of the results. Methods： The serum samples containing different levels of AFP and CEA were collected. External quality control assessment was implemented. The samples were distributed in different laboratories. The participants were requested to perform a conventional assay employed in their laboratories and return their reports in restricted period. The results were classified according to different testing methods and the statistical analysis was performed. Results： Concerning the qualitative assay, the accordance rate was 100% for AFP-negative, -weak positive, and-positive samples; the accordance rate was 100% for CEA-negative samples, 60% for CEA-weak positive samples, and 85% for CEA-positive samples. Concerning the quantitative assay, the coefficient of variation （CV） was in the range of 9.9%-73.9% for AFP and 11.5%-65.3% for CEA. Conclusion ： There was not better comparability of AFP and CEA results measured with different methods. The better comparability required higher standard of quality control.
出处
《肿瘤》
CAS
CSCD
北大核心
2007年第6期490-492,共3页
Tumor
关键词
癌
肝细胞
诊断
甲胎蛋白类
癌胚抗原
诊断技术和方法
Carcinoma, hepatocellular
Diagnosis
alpha-fetoproteins
Carcinoembryonic antigen
Diagnostic techniques and procedures
作者简介
陈慧英（1953-），女（汉族），副主任技师 Correspondence to：CHEN Hui—yin（陈慧英）Tel：021-68316300-2309 E-mail：chenhuiying@sccl．org．cn
