摘要
目的观察移植脊髓源性神经于细胞在臂丛根性撕脱伤后相应脊髓前角的存活、迁移、分化情况。方法取新生鼠脊髓,分离获得脊髓源性神经干细胞,在体外培养、扩增,鉴定,并用5-溴2-脱氧尿苷(Brdu)标记。取SD大鼠20只,将右侧C_5～C_7神经根经后路撕脱,将标记后的神经干细胞移植于损伤侧C_6脊髓前角。术后1、2、4、8、12周取脊髓标本利用免疫组化染色观察神经干细胞的分化情况。结果移植神经干细胞不仅能存活,并可向二端迁移达一个脊髓节段,分化为神经元及星型胶质细胞,其分化趋向呈时间相关性。结论脊髓源性神经干细胞在植入臂丛根性撕脱伤相应脊髓节段前角后不仅能存活和迁移,并能分化为神经元以及星型胶质细胞。
Objective To study the survival, migration and differentiation of the neural stem cells which transplanted into ventral horn of spinal cord after brachial plexus avulsion. Methods Neural stem cells isolated from spinal cord of neogenetic rats and cultured, expanded, labeled by BrdU before transplanted. Twenty adult healthy SD rats preformed as the model of brochial plexus avulsion （ Roots C5-7 ）, then transplanted stem cells into the C6 ventral horn of spinal cord. On 1,2,4,8,12 weeks postoperatively, immunohisto- chemistry assay were carried out in the spinal cord. Results Transplanted into ventral horn of spinal cord after brachial plexus avulsion. Neural stem cells can survive, migrate for at least one segment of spinal cord and differentiate to neurons and astrocytes. The differentiation of stem cells were time-depends. Conclusion Neural stem cells can survive, migrate and differentiate after transplanted into ventral horn of spinal cord in the rats which suffered from brachial plexus avulsion.
出处
《中华显微外科杂志》
CSCD
北大核心
2007年第3期193-196,I0003,共5页
Chinese Journal of Microsurgery
基金
福建省自然科学基金(C0210010)
关键词
臂丛
根性撕脱伤
神经干细胞
前角
脊髓
移植
Brachial plexus
Nerve root avulsions
Neural stem cells
Ventral horn, Spinal cord
Transplantation
