摘要
目的：建立氧氟沙星（OFL）的ELISA快速检测方法。方法：在制备抗OFL单克隆抗体基础上,采用间接竞争酶联免疫吸附方法（ciELISA）检测OFL的含量。结果：筛选得到特异性分泌抗OFL的单克隆抗体细胞株3G3和3D9,应用3G3所制备腹水建立的ciELISA工作曲线的线性范围为0.5-128 ng/mL（r^2=0.985 8）,最低检出浓度为0.5 ng/mL,半数抑制浓度IC50为7.0 ng/mL,样品加样回收率为84%-120%,批内变异系数和批间变异系数均小于15%。结论：本文建立的氧氟沙星ELISA检测法可满足OFL残留检测的需要。
Aim. To develop ELISA for the assay of ofloxacin. Methods： Ofloxacin was determined by indirect competitive inhibition enzyme-linked immunosorbent assay （ciELISA） on the basis of monoclonal antibody against ofloxacin. Results：Two hybridoma lines 3G3 and 3D9 were isolated.The linear range of the ciELISA using 3G3 had a linear range of 0.5 - 128 ng/mL （ r^2 = 0. 985 8）. The low of detection （LOD） of the assay was 0.5 ng/mL, and IC50 was 7.0 ng/mL. The recovery of ofloxacin spiked in samples was among 84% - 120%. Both the inter-assay and intraassay coefficient of variation （CV） was less than 15%. Conclusion： The established method is rapid, sensitive and specificity and could meet the requirements in the determination of ofloxaein residue.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2007年第3期233-235,共3页
Journal of China Pharmaceutical University
基金
河南省杰出人才创新基金资助项目(No.0421002300)~~
作者简介
通讯作者Tel：0371-67763952 E-mail：qulingbo@zzu．edu．cn
