期刊文献+

穿通支原体在宫颈癌患者血液和肿瘤组织中的分离检出 被引量:10

Isolation of Mycoplasma penetrans from blood and tissue specimens of patients with cervical cancer
在线阅读 下载PDF
导出
摘要 目的探讨穿通支原体(Mycoplasma penetrans,Mpe)分离检出与宫颈癌发生的相关性。方法将55例宫颈浸润癌、37例宫颈上皮内瘤变(CIN)及24例对照的宫颈组织、血液标本接种在改良的SP-4、Uu、Mh等液体培养基进行支原体分离培养,可疑阳性菌株用PCR予以证实。MpePCR阳性患者的组织和血液标本超薄切片,电镜下观察Mpe颗粒的存在以及细胞超微结构改变。结果55例宫颈浸润癌患者中共分离到Mpe25例,分离率为45.5%(25/55),37例宫颈CIN患者中共分离到Mpe9例,分离率为24.3%(9/37),24例对照组中有1例从血液和组织同时分离到Mpe(4.2%,1/24),各组Mpe分离率比较均有统计学意义。电镜下发现,Mpe阳性的宫颈鳞状细胞癌患者的血液、组织中均有典型葫芦状、三层膜结构的穿通支原体存在。结论宫颈浸润癌、宫颈CIN患者的组织、血液中Mpe分离阳性率高,提示Mpe感染与宫颈癌的发生有一定关联。 Mycoplasma penetrans was isolated from blood and tissue specimens of 55 patients with cervical cancer, 37 cases with cervical intraepithelial neoplasia (CIN) and 24 normal controls by means of cultivation of the organisms on modified SP-4,Un and Mh liquid media, and the positive isolates were verified by nested polymerase chain reaction (nPGR). The PCR-positive samples of blood and tissues were subjected to be observed under electron microscopy. It was found that the positive detection rate for M. penetrans was 45.5 % (25/55) in patients with cervical cancer and 24.3 % (9/37) in cases with CIN respectively, while it was only 4.2 % (1/24) in normal controls. The observation under transmission electron microscopy showed the flask-shaped morphological feature typical of M. pentrans in blood and tissue specimens. Therefore it is concluded the higher isolation of M. penetrans in blood and tissues of patients with cervical cancer may be associated with the development of this cancer in vivo.
出处 《中国人兽共患病学报》 CAS CSCD 北大核心 2007年第6期537-539,543,共4页 Chinese Journal of Zoonoses
基金 浙江省自然基金资助项目(399066)
关键词 穿通支原体 宫颈肿瘤 Mycoplasma penetrans cervix neoplasms carcinoma
作者简介 通讯作者:周丽萍.Email:zlp@wzmc.net
  • 相关文献

参考文献9

二级参考文献16

  • 1[1]Blanchard A,Montagnier L.AIDS-associated mycoplasmas.Annu Rev Microbiol(J).1994,48:687-712
  • 2[3]Roux KH.Optimization and roubleshooting in PCR.In:Diffenbach CW,Dveksler GS.(eds).PCR Primer:A Laboratory Manual,CSHL Press(M).1995:53 -62
  • 3曹玉璞,支原体与支原体病,2000年,1-6,151-162页
  • 4Lo S C,Lancet,1991年,388卷,1415页
  • 5Brenner C,Neyrolles O,Blanchard A.Mycoplasmas and HIV infection:from epidemiology to their interaction with immune cells.Front Biosci,1996,1:e42-54.
  • 6Zhang S,Wear DJ,Lo S.Mycoplasma infections alter gene expression in cutured human prostatic and cervical epithelial cells.FEMS Immunol Med Microbiol,2000,27:43-50.
  • 7Polianskaia GG,Efremova TE.Effect of mycoplasma contamination of a human cervical carcinoma cell line M HeLa clone 11 on karyotypic variability.Tsitologiia,2000,42:794-801.
  • 8贾成梅 赵季文 糜祖煌 等.在HIV感染者和AIDS患者中首次分离到穿支原体和发酵支原体的报告[J].疾病检测,2001,16:841-842.
  • 9Sasaki Y,Ishikawal J,Yamashita A,et al.The complete genomic sequence of Mycoplasma penetrans,an intracellular bacterial pathogen in humans.Nucleic Acids Res,2002,30:5293-5300.
  • 10Yán ez,A,Cedillo L,Neyrolles O,et al.Mycoplasma penetrans bacteremia and primary antiphospholipid syndrome.Emerg Infect Dis,1999,5:164-167.

共引文献37

同被引文献96

引证文献10

二级引证文献21

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部