摘要
以兰州百合为试材,研究总结出了利用花瓣建立外植体的高频再生体系:用花瓣建立外植体时,灭菌时间控制在15min效果较佳,污染率、失活率均低;适宜诱导花瓣愈伤组织的培养基为MS+BA0.5mg/L+2,4-D 0.5mg/L,诱导率达到90%;适宜芽分化培养基为MS+BA1.5mg/L+NAA0.1mg/L,分化率为75%;适宜继代和增殖的培养基为MS+BA1.5mg/L+IBA0.5mg/L,增殖倍数可达5.8;生根培养基以GS+NAA0.25mg/L最佳,生根率高达100%,试管苗生长势强,且根系发达;用草炭作移栽基质,成活率高,为89.6%。
The technique of micropropagation from petals of Lanzhou Lily was built. The results showed that 15 minutes explants sterilized time had better effect on lower contamination rate,MS＋BA0. 5mg/L＋2,4-D 0.5mg/L was suitable for petal callus induction, MS＋BA1. 5mg/L＋NAA 0. lmg/L was suitable for adventitious shoot formation, the optimum medium for subculture was MS＋BA1. 5mg/L＋IBA0. 5mg/L, and the best medium for root regeneration was GS＋NAA0. 25mg/L with 100% root generation rate and vigorous roots. The living rate of transplanted seedling on pasteurized peat was 89. 6%.
出处
《北方园艺》
CAS
北大核心
2007年第6期210-212,共3页
Northern Horticulture
基金
甘肃省科技攻关资助项目(QS022-C31-47)
关键词
兰州百合
花瓣
诱导
继代
生根
基质
Lanzhou Lily
Petal induction
Subculture
Root regeneration
Medium
作者简介
刘芬（1972-），女，硕士，助理研究员，一直从事植物生物技术研究工作，E-mail：gswzw@163．com。
