摘要
研究了新试剂对甲苯偶氮水杨基荧光酮（MBASF）与钼（Ⅵ）显色反应，以建立水解液中钼的形态分布测定方法。在盐酸介质中，对MBASF与钼（Ⅵ）发生灵敏的显色反应，形成稳定的红色配合物。配合物表观摩尔吸光系数达1．54L·mol^-1·cm^-1，在0～12μg·（25mL）。范围内钼（Ⅵ）服从比耳定律。除常见金属离子外，蛋白、多肽和氨基酸均有高的允许量。大豆酶解液A经等电点沉淀法除去未水解蛋白后得溶液B，溶液B经D301R树脂吸附后，分别采用pH3．6和9．0的缓冲液洗脱分离得溶液C和D；A，B，C和D溶液分别用于光度法测定总钼、水解钼、结合态钼（多肽和氨基酸螯合态钼）和游离钼的含量。
A new method has been developed for spectrophotometric determination of molybdenum modality distributions in soy hydrolysate hydrolyzed by enzymes, based on the study on the color reaction of methybenzeneazosalicylfluorone （abbreviated as MBASF） with molybdenum（ Ⅵ）. In hydrochloric acid medium, MBASF reacts with molybdenum（Ⅵ） sensitively to form a stable red complex, its apparent molar absorptivity is 1.54 L·mol^-1·cm^-1 , and 0-12 μg of molybdenum obeys Beer＇s law in 25 mL of solution. Besides normal metal ions, protein, peptide and amino acid also have high tolerance limits. Soy hydrolysate hydrolyzed by enzyme （A） was precipitated at its isoelectric point, and the filtrate （B） was obtained. Subsequently, B was transferred to the chromatogram column with D301R resin. The column was purged by using 20 mL of HAc-NaAc（pH 3.6） and NH4Cl-NH3·H2O buffer solution（pH 9） orderly and the effluent solutions C and D were obtained. A, B, C and D solutions were used for the spectrophotometric determination of total molybdenum, hydrolyzed molybdenum, chelated molybdenum by peptide or amino acid, and dissociative molybdenum, respectively.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2007年第5期957-960,共4页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(20571033)
石家庄市科技攻关项目(05150142A)资助
作者简介
刘慧珍，女，1981年生，江南大学化学与材料工程学院硕士研究生
通讯联系人e-mail：zaijunli@263．net
