摘要
目的探讨甲基莲心碱(Neferine,Nef)对耐药人乳腺癌细胞增殖,细胞内ADM积聚浓度及mdr-1/P-gp表达的影响。方法采用MTT法测定细胞毒作用,高效液相色谱法测定细胞内ADM积聚浓度,RT-PCR技术及蛋白质印迹技术检测mdr-1/P-gp表达。结果10μg/mlNef+ADM组细胞的抑制率及细胞内ADM积聚浓度比ADM组高(P<0.01);20μg/mlNef+ADM组细胞抑制率及细胞内ADM积聚浓度较5μg/ml异博定+ADM组及10μg/mlNef+ADM组均高(P<0.01)。10μg/mlNef+ADM组MCF-7/Adr细胞mdr-1mRNA及P-gp表达比ADM组明显下降(P<0.01)。20μg/mlNef+ADM组细胞mdr-1mRNA及P-gp表达较10μg/mlNef+ADM组明显下调(P<0.01)。结论Nef能抑制耐药人乳腺癌细胞增殖。Nef能增加MCF-7/Adr细胞内ADM积聚浓度。Nef通过降低耐药人乳腺癌细胞mdr-1mRNA及P-gp的表达逆转MDR。
Objective To investigate the effect of neferine（Nef） on the proliferation, intracellular ADM concentration and the expression of mdr-1/P-gp in human breast cancer cell line MCF-7/Adr, Methods MTT method was used to observe cell proliferation. High performance liquid chromatography was used to detect intracellular ADM concentration. RT-PCR and Western-blotting were used to detect mdr-1/Pgp expression. Results The inhibiting rate and intracellular ADM concentration of 10μg/ml Nef adding ADM group were significantly higher than those of ADM group （P〈0. 01）. The inhibiting rate and intracellular ADM concentration of 20μg/ml Nef adding ADM group were significantly higher than those of 5μg/ml verapamil adding ADM group and 10μg/ml Nef adding ADM group （P〈0.01）. The mdr-1 mRNA and P-gp expression of 10μg/ml Nef adding ADM group was significantly lower than those of 60μg/ ml ADM group（P〈0. 01）. The mdr-1 mRNA and P-gp expression of 20μg/ml Nef adding ADM was significantly lower than that of 10μg/ml Nef adding ADM group group（P〈0. 01 ）. Conclusion Neferine can inhibit the proliferation of human breast cancer cell line MCF-7/Adr. Neferine can improve the intracellular ADM concentration. Neferine can reduce mdr-1 mRNA and P-gp expression and consequently reverse MDR.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2007年第5期351-354,共4页
Cancer Research on Prevention and Treatment
作者简介
黄程辉（1975-），男，博士，主治医师，主要从事恶性肿瘤耐药机制及其逆转研究
