摘要
用玉米油或二月桂酸二丁基锡[DBTD0.01,0.1,1mg/(kg·d)体重]对新生雄性Wistar大鼠连续5d注射染毒.至大鼠出生后第49d,检测其血清睾酮,细胞色素胆固醇侧链裂解酶(P450scc)、3β-羟基类固醇脱氢酶(3β-HSD)和细胞色素P450 17-α脱羟基酶(Cyp17)这3种睾酮合成关键酶mRNA表达及脏器系数.结果表明,大鼠血清睾酮随DBTD剂量升高呈上升趋势,1mg/(kg·d)组与对照组比较有显著性差异;P450scc和3β-HSD mRNA相对表达无显著性变化,Cyp17在0.1mg/(kg·d)组的相对表达有显著性升高;1mg/(kg·d)组大鼠睾丸脏器系数有显著性升高,而各DBTD处理组附睾和肝脏脏器系数与对照组比较无显著性变化.
Newborn male Wistar rats were injected contamination continuously for 5d using di-butyltin dilaurate （DBTD） 0.01,0.1, 1mg/（kg·d） body weight or solvent corn oil. 49d after the birth of rat, its serum testosterone was detected. The mRNAs expressions of three key steroidogenic synthesized enzymes; cytochrome cholesterol side-chain-cleavage enzyme （P450scc）, and 3β-hydroxysteroid dehydrogenase （3β-HSD）, and cytochrome P450 17α-hydroxylase （Cyp17） mRNA, and organ index in rats were detected. The rat serum testosterone tended to rise with the rise of DBTD dosage, 1mg/（kg·d） group compared with the control group rose markedly. The relative expression of P450scc and 3β-HSD mRNA did not change markedly. The relative expression of Cypl 7 in 0. lmg/（kg·d） group rose markedly; the 0. 1mg/（kg·d） group rat testis organ index rose markedly; while each DBTD treating group epididymis and liver organ index compared with the control group had no significant change.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2007年第2期264-268,共5页
China Environmental Science
基金
国家"973"项目(2002CB512908)
作者简介
司纪亮（1973-），男，山东淄博人，山东大学环境科学与工程学院博士研究生，主要从事环境毒理研究．发表论文6篇．
责任作者，教授，hjwsli@sdu．edu．cn
