摘要
目的:对假交替单胞菌JIV-49进行了发酵条件的研究。方法:采用单因素实验法对JIV-49产抗真菌活性物质的发酵培养基及主要的影响因子如初始pH值、温度、培养时间、接种量、转速、摇瓶装液量等进行了考察。结果:确定了最佳培养基为:牛肉膏为2.5%、KBr为0.01%、盐浓度为6%;最佳培养条件为:初始pH值为7.5、温度为30℃、培养时间为96h、接种量为7%,摇床转速为180r/min,摇瓶装液量为75ml/500ml。结论:初步确定了JIV-49发酵的条件,为工业化生产抗真菌活性物质提供了科学依据。
Objective： The fermentation conditions of Pseudoalteromonas sp.JIV-49 were studied.Methods：The fermentation medium and main influence factors of Pseudoalteromonas sp.JIV-49 was investigated with single-factor test.Results： The results showed that the optimal fermentation medium were obtained as follow： NaCl 6%,beef extract 2.5%,potassium bromide 0.01%;the optimal fermentation condition were obtained as follow： initial pH value 7.5,temperauture 30℃,fermentation time 96h,seeding volume 7%,rotated speed 180r/min, 75 ml medium in 500ml flask. Conclusion The establishment of fermentation conditions had provided scientific foundation for industrial production of antifungal activity materials.
出处
《生物技术》
CAS
CSCD
2007年第1期69-72,共4页
Biotechnology
作者简介
詹萍（1979-），女，硕士，助教。从事微生物药物研究，发表论文4篇；
通讯作者：梁静娟（1964-），女，硕士，副教授，从事微生物药物研究工作，E-mail：jjliang@gxu.edu.cn.
