摘要
目的:探讨肝癌对化疗药物的敏感性,筛选个体敏感的化疗药物。方法:采用MTT比色法,对27例原发性肝癌患者癌细胞进行7种抗癌药物敏感性测定。结果:7种化疗药物的敏感率为奥沙利铂(OXA)92.5%,丝裂霉素(MMC)88.88%,顺铂(DDP)81.48%,吉西他滨(GEM)66.66%,表阿霉素(EADM)62.96%,5-氟脲嘧啶(5-FU)51.85%,羟基喜树碱(HCPT)40.74%。结论:MTT法是一种简便、快捷的肿瘤细胞体外药敏试验方法,为肝癌临床个体化治疗提供了实验依据。
Objective.. To study the anti-cancer drug sensitivity in liver cancer and screen the sensitive drugs for individual. Methods： The chemosensitivity of 27 liver cancer samples to 7 chemotherapeutic agents was determined by using MTT assay. Results： Seven chemotherapeutic agents showed sensitive rate of OXA, MMC, DDP,GEM, EADM,5-FU,HCPT were 92. 5%,88. 88%,81. 48%,66. 66%, 62. 96%,51. 85% and 40. 74%, respectively. Conclusion： MTT assay is an easy and quick method for drug sensitivity test in vitro and it provides experimental basis for individual chemotherapy.
出处
《华夏医学》
CAS
2007年第1期39-40,共2页
Acta Medicinae Sinica
关键词
肝癌细胞
药敏试验
MTT比色法
liver cancer
drug sensitivity
MTT colormetric assay
作者简介
覃理灵（1964-），女，2001年桂林医学院临床医学专业专科毕业，现任桂林医学院附属医院肝胆外科研究室主管技师。
