摘要
目的探索尘肺结核患者感染的结核分枝杆菌(MT)L型的耐药基因rpsL突变与耐受链霉素(SM)的关系。方法用PCR-SSCP方法检测rpsL基因,与采用常规SM药敏试验(AST法)的结果进行对比分析。结果采用AST法检测,52株结核分枝杆菌L型临床分离株中共有26株(50.0%)耐SM;PCR-SSCP法检出rpsL基因突变率为40.4%(21/52),2种方法检测耐药株的符合率为80.8%。结论结核杆菌L型对SM的耐药与rpsL基因突变有关。
Objective To study the relationship between mutation of rpsL drug-resistant gene in L-forms of Mycobacterium tuberculosis and drug-resistance to streptomycin in pneumoconiosis patients. Methods A total of 52 clinical isolated strains of Mycobacterium tuberculosis L-forms were collected from 97 pneumoconiosis patients. The mutation of rpsL gene was detected by PCR-SSCP,and the drug-resistance to streptomycin was performed by routine antimicrobial susceptibility test （AST）. Results The results of drug susceptibility test showed that 26 in the 52 clinical isolated strains were drug-resistant to streptomycin. The streptomycln-resistant rate was 50.00% （26/52）. The gene mutation rate of rpsL detected by PCR-SSCP was 40. 38% （21/52）. The coincidence rate of two experimental resuits was 80.77% （21/26）. Conclusion High detectable rate of streptomycin-reslstant strains in Mycobacterium tuberculosis L-forms was found by PCR-SSCP. The application of PCR-SSCP may possess important value for guiding clinical medication of pneumoconiosis patients complicated with tuberculosis among coal workers
出处
《临床检验杂志》
CAS
CSCD
北大核心
2007年第2期107-108,共2页
Chinese Journal of Clinical Laboratory Science
基金
安徽省教育厅自然科学基金项目(2005KJ238)
2005年安徽理工大学青年科学研究基金
关键词
尘肺
结核分枝杆菌L型
耐药
RPSL基因
聚合酶链反应-单链构象多态性分析
pneumoconiosis
Mycobacterium Tuberculosis L-form
drug-resistance
rpsL
polymerase chain reaction and single-strand conformation polymorphism （PCR-SSCP）
作者简介
陆军，1972年生，男，硕士，讲师，主要从事病原生物学检验的教学与科研工作。
