摘要
目的:比较痰菌检查、结明试验、PPD实验和MPT64蛋白抗体检测,评价MPT64蛋白在结核病血清学诊断中的价值。方法:对101例结核病患者进行分析,同时进行痰菌检查、结明试验、PPD实验和MPT64蛋白抗体检测,对结果进行比较对照分析。结果:痰检阳性率20．8％,结明试验45．5％,PPD试验77．2％,PPD抗体79．2％,MPT64抗体74．2％;在肺结核患者,MPT64抗体与PPD试验一致率为75．6％;在胸膜炎患者,MPT抗体检测特异性高达86．7％。结论:PPD试验仍是目前结核病早期诊断与鉴别诊断的重要方法,分泌蛋白MPT64可作为新型结核病快速诊断试剂的组分。
Objective To compare sputum smear, MycoDot TM, PPD and anti-MPT64 antibody assay in tuberculosis patients and evaluate the value of MPT64 protein in serodiagnosis of tuberculosis. Methods Smear, MycoDot TM, PPD and anti-MPT64 antibody assay were used to analyze 101 cases of tuberculosis at equal pace and the results were analyzed statistically. Results The positive rate of smear assay was 20.8%, MycoDot TM assay was 45.5%, PPD test was 77.2%, anti- MPT64 antibody was 74.2%. The concordance rate of anti-MPT64 antibody assay to PPD assay was 75.6% in pulmonary tuberculosis patients. And in tuberculosis pleurisy patients, the specificity of anti-MPT64 antibody assay reached to 86.7%. Conclusion The PPD assay is still the main method for the diagnosis and secreted protein MPT64 may be the composition of new reagent for the rapid diagnosis of tuberculosis.
出处
《实用诊断与治疗杂志》
2007年第4期245-247,共3页
Journal of Practical Diagnosis and Therapy
基金
国家自然科学基金(30470097)
国家自然科学基金(30500432)
关键词
结核病
结核分枝杆菌
分泌蛋白
诊断
Tuberculosis
mycobacterium tuberculosis
secreted protein
diagnosis
作者简介
段艳（1967年-），女，医学硕士，副主任医师，研究方向：结核病诊断方法的改进与新型诊断试剂的研究。
通讯作者：柏银兰，医学博士，讲师，研究方向：结核病新型诊断试剂和新型疫苗的应用研究。Email：amay0001@sina．com．cn。
