摘要
目的:探讨放射性肺损伤大鼠血清细胞因子白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的动态变化。方法:64只雌性Wistar大鼠随机分为照射组(32只)和对照组(32只)。照射组用直线加速器以6 MV的X线照射大鼠右肺,每次3 Gy,每周2次,连续照射5周(累积剂量最高为30 Gy)。于首次照射后5、8、12和26周末分别从两组中各随机抽取8只大鼠,取眶静脉血行血清细胞因子放射免疫学检查。结果:照射组各时间点大鼠血清IL-6、TNF-α水平均较对照组低,其中IL-6在第8周末明显低于对照组(P<0.05),TNF-α在第5周末明显低于对照组(P<0.05),照射组于第12周末时IL-6和TNF-α逐渐恢复,至26周末虽略低于对照组,但差异无显著性。结论:血清细胞因子IL-6、TNF-α在照射后出现不同程度减低,然后逐渐恢复。提示放射性损伤过程中存在全身免疫抑制状态,血清IL-6、TNF-α变化有可能成为放射性肺损伤的预测指标。
Objective： To observe the dynamic changes of serum interleukin - 6 （IL - 6） and tumor necrosis factor -α （TNF -α） in rats with lung injury induced by radiation at different time points after irradiation. Methods： Sixty-four Wistar rats were randomly divided into control group and irradiation group （each n = 32）, which was irradiated at right hemithorax of X ray of 6 MV with a dose of 3 Gy once, twice a week for 5 consecutive weeks （30 Gy totally）. The levels of serum IL - 6 and TNF -α of 8 rats in each group at the end of the 5 th, 8 th, 12 th and 26 th week after the first irradiation were measured by radioimmunoassay （RIA）. Results： The levels of serum IL - 6 and TNF -α in irradiation group were lower than those of the control group at all time points of the observation. The IL - 6 of irradiation group was significantly lower at the end of the 8 th week than that of the control group （P〈0. 05）. The TNF -α was significantly lower at the end of the 5 th week than that of the control group （P〈0.05）. The levels of IL - 6 and TNF-a slowly rised up at the end of the 12 th week, but they were still a little lower than those of the control group, no differences being found between the two groups at the end of the 26 th week. Conclusion.. The levels of serum IL - 6 and TNF-α exhibit a regular reduction after irradiation and afterwards slow rise. It is suggested that the inhibition of body immunity is present in the radiation - induced injury. The changes of serum IL - 6 and TNF -α may become the predictive criteria for the radiation - induced lung injury.
出处
《中国中西医结合急救杂志》
CAS
2007年第2期88-90,共3页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
国家自然基金资助项目(30371826)
作者简介
臧倩（1980-），女（汉族），山东省人，硕士研究生，主要从事肿瘤学的研究（Email；zangqian816@163．com）。
通讯作者：杨明会教授，博士生导师，主任医师（Email：ymh9651@yahoo．com．on）
