摘要
利用Fenton反应、光照核黄素产生活性自由基.OH、.O2-,以分光光度法研究了螺旋藻及螺旋藻多糖体外清除.OH、.O2-的作用。通过FeSO4诱导脂质过氧化模型、硫代巴比妥酸分光光度法研究了螺旋藻及螺旋藻多糖抗脂质过氧化及对.OH引起的DNA氧化损伤的保护作用。结果表明,螺旋藻及螺旋藻多糖能有效清除.OH、.O2-自由基,对脂质过氧化及DNA的.OH氧化损伤有显著抑制作用。
Scavenging effects of Spirulina and polysaccharides Spirulina ptatensts on active oxygen species · OH and · O2^- generated by Fenton reaction and riboflavine photosensitization in vitro were investigated by the means of spectrophotometry. The inhibition effects of Spirulina and polysaccharides Spirulina platensis on -the lipid peroxidation and damage of DNA chain induced by hydroxyl radical were observed by lipid peroxidation system mediated by FeSO4 and thiobarbituric acid spectrophotometry. The results showed that Spirulina and polysaccharides Spirulina platensis could scavenge · OH, ·O2^- efficiently. It could also significantly inhibit lipid peroxidation and damage of DNA by radical.
出处
《化学与生物工程》
CAS
2007年第3期55-57,共3页
Chemistry & Bioengineering
基金
云南省教育厅科学研究基金资助项目(06Y195C)
关键词
螺旋藻
螺旋藻多糖
活性氧
抗氧化
Spirulina platensis
polysaccharides Spirulina platensis
active oxygen
antioxidation
作者简介
李羚（1963-），女，云南会泽人，副教授，主要从事物理化学的教学和研究。
