摘要
为了有效地利用从国内其他地区引进的低蛋白弱筋种质材料,采用SDS-PAGE技术对63份材料进行了高分子量谷蛋白亚基组成的分析。结果表明,参试材料中共有14种HMW-GS类型,Glu-A1位点上有Null、1和2*三种类型,以Null为主(52.4%);Glu-B1位点上有13+16、17+18、7、7+8、7+8+9和7+9六种类型,以7+8为主(55.6%);Glu-D1位点上有10、12、2+12、5+10、5+12五种类型,以2+12为主(61.9%),而5+10为27%。亚基组合类型共有22种,以“1,7+8,2+12”为主(22.2%)。品质评分频率最高的是8分,为38.1%,其次为6分,为14.3%,5分的为9.5%,但品质评分为10分的也有5个材料,频率为7.9%。优质亚基含量等同或高于其他同类研究,品质评分也相对较高,这说明我国弱筋小麦的选择,需要加强对HMW-GS组成的分析。
In order to utilize the weak gluten wheat germplasm collected from other regions in China, the high-molecular-weight gluten subunits （HMW-GS） of the germplasm grown in the Qinghai-Tibet Plateau were analyzed by SDS-PAGE. A total of 14 HMW-GS patterns were identified. At Glu-A1 Loci, three alleles were detected, which encoded subunit 1, 2^＊ and Null, respectively. The frequency of Null was 52.4%. At Glu-B1 Locus, 6 alleles were also detected, which encoded subunits 13＋16, 17＋18, 7, 7＋8, 7＋8＋9 and 7＋9, respectively and 7＋8 was the major subunit pattern（55.6%）. Five alleles encoded subunits 10, 12, 2＋12, 5＋10 and 5＋12, were detected, and 2＋12 was the major subunit pattern （61.9%）. But the frequency of 5＋10 good for bread wheat was 27%. There were 22 subunit combination patterns in these varieties and the major pattern was ＂ Null, 7 ＋ 8, 2 ＋ 12＂ （22.2%）. The frequency of quality score 8 was 38.1%, which was the highest. Quality score 6 was the second （14.3%）, but there were 5 varieties （strains） which had quality score 10 and the frequency was 7.9. The frequencies of high quality HMW-GS were equal to or higher than other reports, and their quality scores were higher relatively. It was indicated that selection of weak gluten strains need analysis of HMW-GS composition.
出处
《西北农业学报》
CAS
CSCD
北大核心
2007年第2期19-23,共5页
Acta Agriculturae Boreali-occidentalis Sinica
基金
中国科学院西部之光联合学者项目
中国科学院知识创新工程项目(KSCX2-SW-304)
中国科学院领域项目(CXLY-2002-6)
关键词
小麦
弱筋
品质
高分子量谷蛋白亚基
Wheat
Weak gluten
Quality
High molecular weight gluten subunit
作者简介
刘宝龙（1980-），男，在读博士，从事小麦遗传育种工作。
通讯作者：张怀刚（1962-），男，研究员，博士，从事小麦遗传育种研究．E—mail：hgzhang@nwipb．ac．cn
