摘要
目的了解P53、GST-π、P170和鼠抗人MDR相关蛋白(MRP)在眼眶腺样囊性癌(ACC)组织中的表达情况。方法免疫组织化学法检测51例眼眶ACC标本和10例正常眼眶组织中P53、GST-π、P170和MRP的表达状况。结果P53、GST-π和P170在眼眶ACC组织中表达阳性率分别为52·94%、100%和82·35%,与眼眶正常组织相比差异有统计学意义。P53和P170在实体型ACC中的表达率明显高于其在腺样-管状型中的表达率;P53在复发组中的表达率明显高于其在初发组中的表达率;各指标之间在表达上无相关性。结论眼眶ACC的多药耐药性与P53、GST-π和P170的过表达有关,但作用机制不尽相同,各指标的检测对于判断预后有参考价值。
Objective Adenoid cystic carcinoma（ACC） is a common malignant tumor in the orbit. In the present study,we investigated the expression and clinical significances of the D53, P-glycoprotein （P170） , glutathione S-transferase （GST-π） and multidrug resistance associated protein（MRP） in orbital ACC to provide the theoretical information and clinical reference. Methods Fifty-one samples of ACC determined by pathohistology were collected, and 10 normal orbital tissue samples were obtained during the operation for other benign orbital tumor. The expression of P53, GST-π, P170 and MRP in orbital ACC and normal orbital tissues was detected by immunohistoehemisry. Results D53,GST-π and PI70 were expressed in orbital ACC, displaying brown-yellow granule in cellular nuclear, cellular membrane and cytoplasm respectively with a expression percentage 52. 94% , 100% and 82.35% respectively, showing a higher expression rate in compared with normal orbital tissues（ 10% , 10% , 30% , X2 = 7. 128, 44. 546, 10. 455, P = 0. 008, 0. 000, 0. 001 ）. The positive rates of P53 and P170 expression in adenoidtubular ACC（30% and 70% ）were lower than in solid ACC（85.71% and 100% , x^2 = 16. 648, 10. 880, P =0. 000, 0. 001 ）. The positive rate of P53 expression in recurrent tumor group was higher than in initial tumor group（84. 62% versus 42.11% , x^2 =7.634, P =0. 006） ;while that of PITO had not significant difference between recurrent tumor group and initial tumor group （P =0. 242）, and GST-π was strongly expressed in all recurrent tumor, initial tumor, adenoid-tubular ACC and solid ACC samples. No MRP was expressed in both ACC tissue and normal orbital tissue. Conclusion The hyperexpression of D53, GST- π and P170 participates in the pathogenesis of acquired MDR in orbital ACC by different pathways. These indexes are of great value in the prognosis evaluation of ACC.
出处
《眼科研究》
CSCD
北大核心
2007年第3期164-167,共4页
Chinese Ophthalmic Research
作者简介
通讯作者：周晓冬（Email：zxdeye@sina.com）
