摘要
目的检测重组日本血吸虫谷胱甘肽-S-转移酶可生物降解微球疫苗免疫小鼠的血清学指标动态变化。方法利用已构建的表达质粒pET28a（＋）-SiGST在E．coli BL21内表达的日本血吸虫谷胱甘肽-S-转移酶融合蛋白，经纯化后的蛋白质以聚乳酸／乙醇酸（PLGA75／25）为包裹材料，制备rSjGST可生物降解微球。按一定剂量，将其单针皮下注射免疫6周龄BALB／c雌性小鼠，分别于免疫后6、9、12、15、18、21周眼眶采血，通过检测血清中特异性IgG、IL-2及IFN-γ的动态水平，观察其免疫效果。结果与对照组相比，各免疫组血清特异性IgG水平在6、9周时差异均有统计学意义（P均〈0．01），弗氏完全佐剂（FCA）组、铝佐剂组均在免疫后9周达峰值，后逐渐下降，而微球组在21周时仍呈上升趋势（P〈0．01）；微球组、FCA组及铝佐剂组IL-2、IFN-γ水平在6、9周时差异均有统计学意义（P分别〈0．05、0．01、0．01），但微球组在12～21周时仍呈上升趋势（P〈0．01），FCA组、铝佐剂组则在12周后逐渐下降。微球组分别与FCA组和铝佐剂组间比较，微球组血清特异性IgG水平在12周后、IL-2在18周后及IFN-γ在15周后差异均有统计学意义（P均〈0．01）。结论重组日本血吸虫谷胱甘肽-S-转移酶可生物降解微球免疫小鼠，具有明显长效和高效作用。
Objective To detect the dynamic changes of antibody and eytokines in the serum induced by single vaccine delivery system （SVDS） of biodegradable mierospheres of recombinant glutathione-S-transferase from Schistosoma japonicum （rSiGST） in mice. Methods By using the constructed expression plasmid pET28a（＋）-SjGST, rSjGST was expressed by E. coli BL21. After purified through the column of resin,the biodegradable mierospheres of rSjGST were prepared with PLGA （Poly laetie-eo-glyeolie acid, PLGA 75/25）. Six-week BALB/c female mice were immunized subcutaneously with a single vaccine, and the blood was collected from eyes on the 6th, 9th, 12th, 15 th, 18th, 21th week respectively. The dynamic levels of serum specific antibody IgG, IL-2 and IFN-γ were detected. Results Compared with the control group, there was a significant difference of serum specific antibody IgG of every immune group （P〈0. 01）. In FCA and Al-adjuvant groups, the antibody IgG reached the peak at the 9th week, and then descend gradually, but in the microsphere group it increased at the 21th week （P〈0. 01）; there were significant differences on IL-2 and IFN-γ in microsphere group （P〈0. 05）, FCA group （P〈0.01） and Al-adjuvant group （P〈 0.01） at the 6th, 9th week, but in the microsphere group they increased consistently （P〈0.01） from the 12th to 21th week, and in FCA and Al-adjuvant groups they both descended gradually after the 12th week. While the comparison was made between the microsphere group and FCA group as well as the microsphere group and Al-adjuvant group respectively, there were significant differences of serum specific antibody IgG after the 12th week, IL-2 after the 18th week, and IFN-γ after the 15th week in the microsphere group（P〈0. 01）. Conclusion There is a longer and higher effect induced by biodegradable microspheres of rSjGST in mice, which provides a new means for further study on a vaccine of Schistosorna japonicurn.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
2007年第2期98-101,共4页
Chinese Journal of Schistosomiasis Control
基金
安徽省教育厅自然科研基金(2005kj303)
作者简介
唐小牛（1967-），男，硕士，副教授。研究方向：血吸虫分子免疫学
