摘要
目的研究异亚丙基莽草酸（ISA）体外对过氧化氢（H2O2）诱导人脐静脉内皮细胞（HU—VEC）脂质过氧化的影响。方法采用硫代巴比妥酸微量法测定丙二醛（MDA）；黄嘌呤氧化酶法测总超氧化物歧化酶（SOD）；可见光法测过氧化氢酶（CAT）。采用体外自由基捕捉实验检测ISA对超氧阴离子和羟自由基的清除率。结果预先用ISA10^-5、10^-4mol／L培养HUVEC6h，H2O2（200μmol／L，6h）所致的细胞膜脂质过氧化反应明显减弱，MDA产生减少，CAT和SOD活性受到保护。但仅以ISA孵育HUVEC12h，对SOD和CAT活性没有直接的影响。ISA10^-5-10^-3mol／L对超氧阴离子有明显的清除效果，并呈现出量效关系。ISA（10^-3mol／L）对羟自由基也有一定的清除作用。结论ISA体外对血管内皮细胞具有抗脂质过氧化作用，对抗氧化酶SOD和CAT的活性无直接影响。
Objective To study the influence of 3, 4 -oxo-isopropylidene -shikimic acid （ISA） on lipid peroxidation of human umbilical vein endothelial cells （HUVEC） induced by hydrogen peroxide （H202 ） in vitro. Methods The concentration of malonaldehyde （MDA） in HUVEC was detected by thiobarbituric acid （TBA） method, the activities of superoxide dismutase （SOD） was detected by xanthine oxidase system and catalase （CAT） was detected by visible spectrum assay. The clearance （C） of superoxide anion and OH radicals by ISA were tested by free radical prehensile experiment in vitro. Results The lipid peroxidation induced by H2O2（200 μmol/L for 6 hours） was alleviated obviously in HUVEC cultured with ISA （ 10^-5, 10^-4 mol/L） for 6 hours, MDA was decreased and the activities of CAT and SOD were protected. The activities of SOD and CAT were improved after HUVEC incubation with ISA for 12 hours. ISA （ 10^-5 - 10^-3 mol/L） had an obvious clearing effect on superoxide anion, showed a relationship between quality and effectiveness. ISA （10^-3 mol/L） could also clear OH radicals. Conclusion ISA has an antagonizing effect on lipid peroxidation of vein endothelial cells in vitro, but no direct influence on the activities of SOD and CAT.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2007年第2期98-100,104,共4页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家科技部"十五"科技攻关项目(No2001BA701A07-14)
关键词
异亚丙基莽草酸血管内皮
细胞
过氧化氢
3, 4 -oxo-isopropylidene-shikimic acid
vein endothelial cells
hydrogen peroxide
作者简介
马怡，女，博士
通讯作者：孙建宁，女，教授，博士生导师
