摘要
目的观察高效抗逆转录病毒治疗(HAART)前后人类免疫缺陷病毒Ⅰ型(HIV-1)慢性感染儿童外周血HIV-1特异性CTL的反应特点,并分析其影响因素。方法采集24例HIV-1慢性感染儿童外周血并分离PBMCs,常规进行CD4T细胞绝对数和血浆病毒载量测定。应用tetramer染色技术和ELISPOT技术分别测定HIV-1表位特异性CTL频率和IFN-γ产生细胞的数量。结果在80%的HIV-1慢性感染儿童外周血可检测到HIV-1特异性的tetramer+CTL细胞和HIV-1表位肽刺激的IFN-γ产生细胞。未经HAART治疗和HAART治疗失败的儿童,其体内CTL反应明显强于HAART治疗成功的儿童,并且这种CTL反应与HAART治疗时间呈明显的负相关。结论HAART治疗后外围血HIV-1特异性CTL反应明显降低,持续的HIV-1抗原刺激是维持外周血HIV-1特异性CTL反应的主要因素。
Objective To characterize the properties of human immunodeficiency virus-1- （HIV-1） specific cytotoxic T lymphocyte （CTL） responses, and to analyze the factors which may affect CTL responses before and after treatment with highly active antiretroviral therapy （HAART） in children with chronic HIV-1 infection. Methods A total of 24 HIV-1-infected children were studied. Circulating CD4 T cell counts and HIV-1 RNA levels were routinely measured. Peripheral HIV-1-specific CTL frequency and the specific interferon-γ- producing lymphocytes were determined by tetramer staining and enzyme-linked immunospot （ELISPOT） assay, respectively. Results It was found that HIV-1-specific CTL responses could be detected in vitro in more than 80% of Chinese children with HIV-1 infection. Circulating CTL, frequency and IFN-γ-producing lymphocyte counts were significantly reduced in HAART suppressed HIV-1 infected children in comparison with HAART failure children and HAART na？ ve children. The duration of virus suppression after HAA-RT was found to be inversely correlated with CTL frequency. Conclusion HIV-1-specific CTL response to virus was warranted by antigenic stimulation. The present work may elucidate the immune mechanisms influencing the outcome of HIV-1 carriers with treatment of HAART.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2007年第2期107-109,共3页
Medical Journal of Chinese People's Liberation Army
作者简介
金磊，主管技师。主要从事分子生物学和免疫学基础研究。已发表论文30余篇，获军队和北京市科技进步奖4项
【通讯作者】王福生，E-mail：fswang@public.bta.net.cn
