摘要
目的建立参芪扶正总苷的高效液相色谱指纹图谱。方法Waters 717型高效液相色谱仪,Waters2996二极管阵列检测器,低压四元泵,自动进样器;KR100-5C18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-水,线性梯度洗脱;柱温:30℃;流速:0.8 mL.min-1;检测波长:270 nm;记录时间:90min。结果10批样品与对照用参芪扶正总苷高效液相色谱指纹图谱的相似度均>90%,精密度、稳定性、重复性实验中各共有峰相对保留时间和相对峰面积RSD均<3%。结论该方法操作简便,结果稳定可靠,可作为参芪扶正总苷的质量控制方法。
ABSTRACT Objective To set up an HPLC fingerprint of the total saponins in shenqifuzheng injection. Method The devices used were Waters 717 high performance liquid chromatograph, Waters 2996 diode array detector, low pressure quatemionic pump, autosampler and KR100-5C18 chromatographic column （250mm×4.6mm,5μm）. The mobile phase was acetonitrile-water, using linear gradient elution, the column temperature was 30 ℃ ; flow rate, 0. 8 mL·min^-1; detection wavelength, 270 nm; proceeding time, 90 minutes. Results The fingerprints of all the 10 batches of samples showed a greater than 90% similarity to those of total saponins in the control shenqifuzheng injection. The RSD of the relative retention time as well as that of the relative peak area of the shared peak was lower than 3% in the precision, stability and reproducibility tests. Conclusion The method was shown to be handy and reliable and may be used in the quality control of the total saponins in shenqifuzheng injection.
出处
《医药导报》
CAS
2007年第2期187-189,共3页
Herald of Medicine
基金
*湖北省科技攻关项目(基金编号:2006AA301B16-4)
作者简介
张勇慧（1972-），男，湖北浠水人，副教授．博士，主要从事天然药物生物活性成分及中药新药的开发研究。电话：027-83657870，E—mail：zhangyh@mails．tjmu．edu．cn。
[通迅作者]吴继洲，男，博士，博士生导师，电话：027-83692739，E-mail：ywjz@mails．tjmu．edu．cn。
