摘要
以青皮果品种的叶片、叶柄和无芽茎段作为外植体,研究其直接分化再生苗能力的差异。结果表明:⑴MS+6-BA1.0mg/L+IAA0.1mg/L和MS+6-BA2.0mg/L+IAA0.1mg/L两组培养基均可使叶片外植体直接分化出芽并建成再生苗。叶片基部的分化能力最强,中部稍次,尖部最弱。⑵MS+6-BA3.0mg/L+IAA0.1mg/L则诱导叶片外植体首先脱分化形成愈伤组织,继而再分化成再生苗,,且畸形苗居多,无应用价值。⑶叶柄和无芽茎段在三组培养基中都只能脱分化形成大量愈伤组织,难以分化再生苗。
Leaf, petiole and stem cuttings（without buds） of Siraitia grosvenorii were used as explants,in order to study their differentiate abilities of regenerate plantlet in vitro. The results showed that ： （ 1 ） On medium MS ＋ 6-BA 1.0 mg/L＋ IAA0.1 mg/ Land MS ＋ 6-BA2.0 mg/ L ＋ IAA0.1 mg/ L, regenerated plantlets could be directly induced from the segments of leaf. The base part segments of Leaf showed strongest ability in differentiating regenerated plantlets, then was the medium part segments and last was the tip part segments. （2） Medium MS ＋6-BA 3.0 mg/ L ＋ IAA 0.1 mg / L induced firstly the segments of leaf to form callus ,and then the callus differentiated regenerate plantlets, but most of the plantlets were deformity. （3） Petioles and stem cuttings （ without buds） easily formed a great quantity of callus on the above three media, but difficult to form regenerate plantlets.
出处
《生物技术通报》
CAS
CSCD
2006年第C00期514-516,533,共4页
Biotechnology Bulletin
基金
广西教育厅植物学硕士点重点建设项目(D-1008)资助
关键词
罗汉果
再生苗
器内培养
分化能力
Siraitia grosvenorii Regenerated plantlets Culture in vitro
作者简介
蓝桃菊（1980-），女，广西来宾市人，广西大学农学院硕士研究生。
通讯作者：许鸿源，地址：广西大学东校院6988信箱，邮编530005．E-mail：xuhy08@126．com．Tel．0771-3238464。
