摘要
目的：了解急性早幼粒细胞白血病（APL）患者外周血TCRVα亚家族T细胞的分布及其克隆性。方法：利用RT-PCR扩增9例APL患者和10例正常人外周血单个核细胞中29个TCRVα亚家族基因的互补决定区3（CDR3），了解TCRV理亚家族T细胞的分布；阳性产物进一步经基因扫描分析CDR3长度，从而了解其克隆性。结果：9例患者外周血T细胞所能检测到的Vα亚家族T细胞分别为1～9个亚家族，以Vet3和Vα19出现频率较高；克隆性增殖T细胞可在8例患者中检测到，以Vα3、Vα26和Vα27出现克隆性T细胞频率较高（3／8）。正常人外周血T细胞中表达绝大多数Vα亚家族，呈多克隆性增殖特点。结论：APL患者外周血Vα亚家族T细胞存在明显的倾斜性分布和克隆性增殖特点，后者可能是对APL细胞抗原的反应性增殖，是否具有特异性杀伤作用，有待进一步检测。
Objective：To investigate the distribution and clonality of TCR Vα subfamily T cells in patients with acute promyelocytic leukemia （APL）. Methods： The complementary determining region 3 （ CDR3 ） of TCR Vα 29 subfamily genes in peripheral blood mononuclear cells from 9 APL patients were amplified using RT-PCR. The positive products were further analyzed to identify the clonality of T cells by Genescan. Results： 1-9 subfamilies could be detected in peripheral blood T ceils from 9 cases with APL, the fre- quent expression of Vα subfamilies predominated in Vα3 and Vα19. Clonal expanded T cells could be detected in 8 APL patients, which predominant used Vα3, Vα26 or Vα27 （3 out of 8 cases）. However, almost all Vα subfamilies with polyclonal expansion could be detected in Peripheral blood T cells from 10 cases of normal individuals. Conclusion：Remarkable skew distribution and clonal expansion of TCR Vα subfamilies T cells are the common feature in patients with APL. Clonal expansion of T cells might reflect a re- sponse in host to APL cell associated antigen, whether these expanded T cells have the ability for specific cytotoxicity against APL cells, remains an open question.
出处
《临床肿瘤学杂志》
CAS
2007年第2期81-84,共4页
Chinese Clinical Oncology
基金
国家自然科学基金项目(39870358)
广东省科技计划项目(2005B50301016)
广州市科技计划项目(2003J1-10011
200521-E4015)
血液病学国务院侨办重点学科基金资助项目
作者简介
通讯作者。E-mail：jnyangqiuli@163．com
