摘要
目的:探讨肾素-血管紧张素系统基因中血管紧张素原(AGT)基因M235T多态、血管紧张素Ⅰ转换酶(ACE)基因插入/缺失(I/D)多态和血管紧张素Ⅱ-1型受体(AT1R)基因A1166C多态与子痫前期的关系。方法:应用聚合酶链反应(PCR)、限制性酶切及电泳分型等方法对45例子痫前期患者(子痫前期组)和45例非高血压妊娠妇女(对照组)的AGT基因M235T多态、ACE基因I/D多态、AT1R基因A1166C多态性进行分析。结果:(1)AGTM235T多态的TT基因频率两组比较差异有显著性。(2)ACE基因DD、ID、II型在两组中分布无明显差异。(3)AT1R基因A1166CAA、AC、CC型在两组中分布无明显差异。(4)子痫前期组具备TT基因型者,其合并DD基因型的95%CI为1.473～31.919,合并1166C等位基因C的95%CI为1.316～18.991。结论:AGT变异基因235T与子痫前期发生有关,T等位基因可能是子痫前期的易感基因。ACE基因DD型与子痫前期的相关性限于携带有M235T等位基因的患者。携带有AT1R基因C等位基因和AGT基因TT型的妇女子痫前期发病危险升高。
Objective To investigate the relationship between preeelampsia and the polymorphisms of angiotensinogen （AGT） gene M235T, angiotensin Ⅱ type 1 receptor（AT1R）gene Al166C,and angiotensin-1 eomverfing enzyme（ACE） gene insertion/deletion（I/D） in the rennin-angiotensin system. Methods We used polymerase ehain reaetion（PCR）, restriction enzyme, and eleetrophoresis to analyze the polymorphisms of AGT gene M235T, AT1R gene A1166C, and ACE gene I/D in 45 patients with preeelampsia （preeelampsia group） and 45 non-hypertensive pregnant females （eontrol group）.Results A significant difference in genotype TT of AGT M235T polymorphism was found between the preeclampsia group and the control group（P 〈 0.05）. There was no differenee in genotypes DD, ID and II of ACE gene（P 〉 0.25）. Little difference in genotypes AA, AC and CC of ATIR Al166C was found （P 〉 0.25）. The preeelampsie patients with genotype ＇IT would jointly have genotype DD （95%CI 1.473-31.919）, or have the allele of A1166C （95%CI 1.316~18.991）. Conclusions AGT mutant 235T gene is associated with the pathogenesis of preeelampsia and its T allele may be a susceptible factor for preeelampsia. Only the patients carrying the allele M235T have correlation between genotype DD of ACE and preeelampsia.There is an inerease in the risk for the occurrence of preeclampsia in the females who have allele C AT1R gene and genotype Tr of AGT gene.
出处
《实用医学杂志》
CAS
2007年第3期354-357,共4页
The Journal of Practical Medicine
作者简介
通讯作者：王泽华E-mail：zehuawang@163．net
