摘要
目的分析鲍曼不动杆菌的耐药性及其产OXA-23型碳青霉烯酶基因的核苷酸序列。方法用自动微生物分析仪测定常用抗菌药的M IC50;对OXA-23型碳青霉烯酶基因进行PCR扩增,产物纯化测序。结果15株产ESBL s鲍曼不动杆菌中有12株携带OXA-23型碳青霉烯酶;PCR产物纯化后测序表明与鲍曼不动杆菌(AY 795964.1)blaOXA-23基因序列100%同源。结论携带OXA-23型碳青霉烯酶基因的鲍曼不动杆菌对临床常用抗菌药的耐药率高,其编码基因为blaOXA-23。
emase-encoding ge Objective To analyze drug resistance of Acineiobacter baumannii and it＇s OXA-23 carbapenne. Methods PCR amplification was performed of OXA-23 carbapenemase-encoding gene plasmid by PCR, PCR products purifing and nucleotide sequencing. Results There were 12 OXA-23 carbapenemase-producing strains in 15 isolates of ESBLs （＋） A. baumannii. Sequencing analysis of this product revealed 100% identity with blaoxA.23 which was originally detected in another strain of A. baumannii （AY795964. 1）. Conclusion An isolate of Acinetobacter baumannli producing OXA-23 carbarpenase was highly resistant to commonly use antibiotics and it＇s encoding gene is blaoxA-23.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2007年第2期123-124,127,共3页
Chinese Journal of Antibiotics
基金
江西省卫生厅课题(20043143)
作者简介
李蓉，女，生于1973年．博士，副教授。研究方向;细菌耐药机制。
通讯作者：E—mail：liwenlin999@sina．com
