摘要
目的探讨鼻及咽部原发性非霍奇金淋巴瘤(NHL)的临床特点、免疫表型及其与 EB病毒感染的关系。方法对158例鼻及咽部原发性 NHL 进行了 HE 和免疫组织化学 SP 法(CD3、CD20、CD56、CD57)检查,按 WHO 2001年《造血和淋巴组织肿瘤的病理学和遗传学》标准进行分类;并对其中99例进行了 EBER-1原位杂交的检测。结果 158例鼻及咽部原发性 NHL 中,原发于鼻腔84例(53.2%),扁桃体39例(24.7%),咽部35例(22.1%)。结外鼻型 NK/T 细胞淋巴瘤101例(63.9%)、非特异性外周 T 细胞淋巴瘤23例(14.6%),B 细胞淋巴瘤34例(21.5%)。99例 EBER-1原位杂交结果显示阳性率:结外鼻型 NK/T 细胞淋巴瘤为98.6%(70/71),而非特异性外周 T 细胞淋巴瘤为66.7%(8/12),B 细胞淋巴瘤为43.8%(7/16)。结论鼻及咽部 NHL 中结外鼻型 NK/T 细胞淋巴瘤最为多见,与 EB 病毒密切相关,其病理诊断需结合其免疫表型特征及肿瘤部位。
Objective To study the clinical features, immunophenotypes and the significance of Epstein-Barr virus infection in primary nasal and pharyngeal non-Hodgkin＇s lymphomas in Shenyang. Methods One hundred and fifty eight cases of primary nasal and pharyngeal non-Hodgkin＇s lymphomas were included in this study. The samples were stained with haematoxylin and eosin for histological examination. try studies were performed using monoclonal antibodies, including CD3 for T-lymphocytes, CD20 for B-lymphocytes, and CD56 and CD57 for NK cells. All cases were reclassified according to the new WHO classification of lymphomas （ 2001 ）. In situ hybridization detection of EBV- encoded small nuclear RNA （ EBER-1 ） was performed in 99 cases. Results Overall, 101 （63.9%） of the 158 NHL were extranodal NK/T cell lymphomas （nasal type）, 23 （14. 6% ） were nonspecific peripheral T cell lymphomas and the remaining 34 cases （21.5%） were B cell lymphomas. The primary sites of involvement were the nasal cavity （ 53.2% , 84/158 ）, the tonsil （ 24. 7%, 39/158 ） and the pharynx （22. 1%, 35/158）. Among 99 cases studied by EBER-1 in situ hybridization, a positive detection was seen in 70/71 cases （98. 6% ） of extranodal NK/T cell lymphoma （ nasal type）, 8/12 cases （66. 7% ） of T cell lymphoma, and 7/16 cases （43.8%） of B cell lymphoma. Conclusions Among primary nasal and pharyngeal NK lymphomas, extranodal NK/T cell lymphoma （ nasal type ） is the most common type and is strongly associated with EBV infection. The pathological diagnosis of nasal and pharyngeal lymphomas should take considerations of the anatomic sites and immunophenotypical features.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
2007年第2期94-97,共4页
Chinese Journal of Pathology
基金
日本学术振兴会基金[B(2)13576003
B16406006]
关键词
鼻咽肿瘤
淋巴瘤
T细胞
非霍奇金
疱疹病毒4型
原位杂交
Nasopharyngeal neoplasms
Lymphoma, T cell
Lymphoma, non-Hodgkin
Herpesvirus 4,human
In situ hybridization
作者简介
通信作者：贾心善（E-mail：xinshanjia@hotmail．com）。
