摘要
通过农杆菌和直接DNA转移技术所获得的转基因植株都具有复杂的转基因座位,转基因整合染色体和染色体区段是随机的,但组织培养的选择作用表现为非随机性,偏向整合于染色体的基因富集区。转基因座位除少数含有完整的单拷贝转基因之外,大多数转基因座位中外源转基因片段、基因组片段和填充DNA相间而存在。转基因座位中转基因及基因组DNA片段产生缺失、重复和染色体的重排,转基因的完整性对转基因表达具有重要作用。
The plants transformed by either Agrobacterium-mediated or directly delivered DNA transformation possess complex transgenic loci. The transgenes tend to be integrated into gent-rich regions on chromosomes due to selection of transgenic cells during tissue culture rather than integrated elsewhere randomly. Fragments of transferred DNA are interspersed with genomic DNA and filler DNA at most of transgenic loci except a few which contain complete single-copy of transgene. Analysis of transgenic loci flanking sequences indicated that deletion, duplication and chromosomal rearrangement often occurred for transgene and genomic DNA. The complete transgenic structure is important for its expression in transformed plants.
出处
《遗传》
CAS
CSCD
北大核心
2007年第2期157-162,共6页
Hereditas（Beijing)
基金
国家自然科学基金项目资助(编号:30471413)~~
关键词
农杆菌介导
直接DNA转化
转基因座位
染色体重排
旁侧序列
Agrobacterium-mediated
directly delivered DNA
transgenic locus
chromosomal rearrangement
flanking sequences
作者简介
陈建民（1954-），男，江苏如皋人，教授，博士，研究方向：作物分子细胞遗传。Tel：0514-7979286；E-mail：jmchen@yzu．edu．cn
