摘要
啤酒酿造过程中,啤酒腐败菌的检测一直采用传统的培养基检测。随着啤酒工业的迅猛发展,寻求一种快速、简便的检测方法是必然的要求。目前快速检测方法的研究主要表现在三个方面:(1)ATP生物发光检测方法,该方法已经应用于一些食品行业的公共卫生检测和微生物质量控制,而在啤酒工业,它适用于清酒和成品啤酒的微生物在线检测,最大优点是快速、简单和高灵敏度。(2)免疫学的检测方法适用于啤酒酿造过程的各个阶段的样品,其专一、简便、易于自动化的特点预示其有较好的发展前景,但需要降低单克隆抗体的制备成本。(3)基于核酸的分子生物学检测方法有PCR及其衍生技术,它们的优点是专一性强,灵敏度高,适于应急生产中出现的突发事件,但缺点是检测费用高,另外不能区分死菌和活菌。
The method for beer spoilage detection all has been adopted the conventional plate culture method in brewing industry. Nowadays, a rapid and simple detection method was required urgently with the brewing development. The research was focused on three fields： （1） ATP bioluminescence technique is increasingly used for hygiene control and microbial quality control in food industry and adopted to detect the spoilage of clear beer and finished beer for its rapid, simple and highly sensitive characteristics. （2） Immunoassay is a suitable method for detecting different samples of fermenting process. This method is specific, simple and easy for automation which in possible rapid detection of beer spoilages while the cost of producing monoclonal antibodies can be reduced greatly. （3） There is important detection development in the use of nucleic acid-based assays, especially with aspect to PCR techniques. Although PCR method was strongly specific and highly sensitive to be preferred by breweries, there are also a few problems such as quantification and distinguishing of dead cells from viable ones.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第1期360-366,共7页
Food Science
关键词
快速检测方法
ATP生物发光
免疫学方法
PCR
啤酒腐败菌
rapid detection method
ATP（adenosme triphosphate） bioluminescence
immunoassay
PCR（polymerase chain reaction）
beer spoilage
作者简介
朱林江（1981-），男，硕士研究生，主要从事啤酒酿造微生物的研究。
通讯作者
