摘要
目的:采用MTT法测定不同分化程度胃腺癌细胞株对紫杉醇、奥沙利铂的敏感性。方法:以不同浓度的紫杉醇、奥沙利铂分别处理MKN45(低分化)和SGC7901(中分化)2种胃腺癌细胞株,采用MTT法检测紫杉醇、奥沙利铂对胃腺癌细胞增殖的影响。结果:紫杉醇、奥沙利铂分别对2株胃腺癌细胞的增殖均具有显著的抑制作用,中分化的SGC7901细胞株对2种化疗药物的敏感性明显高于低分化的MKN45细胞株,而低分化的MKN45细胞株对紫杉醇更敏感。结论:不同分化程度的胃腺癌细胞株对紫杉醇、奥沙利铂敏感性不同。
OBJECTIVE：To determine the sensitivity of paclitaxel or oxaliplatin in gastric cancer celllines by MTT assay. METHODS： Two gastric cancer cell lines included MKN45（low degree differentiation） and SGC7901（moderate degree differentiation） were incubated with paclitaxel or oxaliplatin of various concentration.The cytotoxicity was examined by MTT test. RESULTS：Paclitaxel or oxaliplatin had a significant killing effect on human gastric cancer cells in vitro, gastric cancer cell line SGC7901 is more sensitivity to chemotherapy than poorly differentiated gastric cancer cell line MKN45, which is more sensitivity to paclitaxel than oxaliplatin .CONCLUSIONS： The effect of paclitaxel or oxaliplatin on gastric cancer cells was different among 2 celllines.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第4期267-268,共2页
China Pharmacy
关键词
紫杉醇
奥沙利铂
分化程度
胃腺癌细胞株
敏感性
MTT法
Paclitaxel
Oxaliplatin
Degrees of differentiation
Gastric cancer cell lines
Sensitivity
MTT methods
作者简介
主管药师。研究方向：临床药学。电话：010—66937405。E-mail：w4244@sina．com.
