摘要
目的:建立一种快速准确同时测定丹参片中脂溶性及水溶性有效成分的方法。方法:建立PR-HPLC法,以甲醇-0.5%甲酸水溶液为流动相进行梯度洗脱,丹参素、丹酚酸B、隐丹参酮、丹参酮ⅡA在Diamonsil C18(250mm×4.6mmID,5μm)柱上得到完全分离。结果:4种物质线性良好,回收率在96.63%～100.3%之间且RSD小于2%。
Aim： To develop a method for rapid and sensitive simultaneous determination of the hydrophihc and lipophihc active components in Salvia miltiorrhiza tablets. Methods： RP-HPLC condition was established to separate dansheusu, salvianolic acid B, cryptotanshinone and tanshinone Ⅱ A from each other on the Diamousil C18 （250 mm × 4.6 mm ID,5μm） column with the mobile phase consisted of methanol and 0.5% formic acid in gradient elution. Results： The four active components had good linearity. The average recoveries ranged between 96.63% to 100.3% with RSDs of less than 2%. Conclusion： The method appeared to be precise and accurate for the simultaneous determination of the four active components in Salvia miltiorrhiza Bunge tablets within 35 min.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2007年第1期51-54,共4页
Journal of China Pharmaceutical University
作者简介
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