摘要
将鸡传染性法氏囊病病毒TS株VP2基因置于植物组成型表达启动子CaMV 35S之下,构建了IBDV VP2基因的表达载体pBR-VP2,经根瘤农杆菌介导法将VP2基因整合到烟草基因组中,Northern杂交结果表明,转基因烟草中存在IBDV VP2基因的mRNA;Dot-ELISA和Western blotting检测表明IB-DV VP2基因在烟草中得到了表达。
Introduction of the VP2 gene of IBDV TS strain under control of CaMV 35S promoter resulted in the construction of plant expression vector pBR-VP2. IBDV VP2 gene was integrated into the plant genomic DNA via Agrobacterium tume.faciens-mediated transformation. Northern blot analysis indicated that the foreign IBDV VP2 gene was correctly transcripted into mRNA. The protein IBDV VP2 expressed in transgenic tobacco was detected by Dot-ELISA and Western blot.
出处
《动物医学进展》
CSCD
2007年第2期19-21,共3页
Progress In Veterinary Medicine
作者简介
杨龙峰（1980-）男，山东人，硕士研究生．主要从事分子病理学研究。
通讯作者
