摘要
从临床怀疑为牛病毒性腹泻病毒(BVDV)感染奶牛的血液中用RT-PCR方法检测BVDV,目的片段回收克隆测序,经Blast分析,与匈牙利疫苗致弱株同源性99%,并用PCR方法鉴定基因型,均为BVDV-Ⅰ型。用MDBK细胞鉴定生物型,不致细胞病变,为非致细胞病变(nCP)型。
BVDV were detected from the blood samples of dairy cow infected suspiciously with BVDV by RT-PCR method. The goal fragment was cloned and sequenced. It shared 99% homology with Hungarian vaccine strain. The gene type was identified as BVDV-1 type by PCR. The biological type was identified as non-cell pathogenic type by using MDBK cells.
出处
《动物医学进展》
CSCD
2007年第1期50-52,共3页
Progress In Veterinary Medicine
关键词
牛病毒性腹泻病毒
非致细胞病变
Bovine viral diarrhoea virus
non-cell pathogenicity
作者简介
郭燕（1979-），女，山东费县人，硕士研究生，主要从事动物传染病的诊断防制。
通讯作者
