摘要
目的：建立槐果碱的LC—MS测定法，研究其在Caeo-2细胞上的转运特征。方法：采用Zorbax Extend-C18柱，流动相10mmol·L^-1醋酸铵水溶液-乙腈（85：15），流速0．2mL·min^-1。电喷雾离子化（ESI）方式，采用选择性离子检测法，检测离子为正离子，槐果碱的选择性离子为[m／z＋H]^＋247．3；考察了温度、药物浓度、时间对槐果碱在Caco-2细胞上转运的影响。结果：槐果碱在5—2000μg·L^-1呈良好的线性关系（r=0．9985），最低定量限为5μg·L^-1；槐果碱在Millicell系统上的转运量具有一定的浓度-时间依赖性，其转运表现为被动扩散，槐果碱的平均表观通透系数Papp为3．159×10^-5cm·s^-1。结论：该法灵敏、简单，专属性强，槐果碱在Caeo-2细胞上具有良好的转运。
Objective： To establish LC-MS method for determination of sophocarpine （SC） and investigate its transport in millicell system. Method： Separation of SC was carried out by an extend-C18s column （2.1 mm × 50 mm, 5 μm, agilent technologies, USA）. The mobile phase was acetonitrile-water containing 10 mmol ·L^-1 ammonium acetate （15：85 ） at the flow rate of 0. 2 mL·min^-1. The ESI ion source was set in the positive ion polarity mode for acquiring all mass spectrometry data. The selective ion monitoring （SIM） was used, and protonated molecules [ M ＋ H ]^＋ were detected at m/z 247.3 for SC. This LC-MS method was applied to determine SC concentration in millicell system. Result： The linearity of SC ranged from 5 to 2 000 μg ·L^- 1（ r = 0. 998 5 ）. The quantification limit of SC was 5 μg ·L^-1. SC transport in Caco-2 cells was time and concentration dependent, and SC transport complied with the passive diffusion. Its average apparent permeability coefficient （P,pp） is 3. 159 × 10^-5 cm·s^-1. Conclusion： This assay was highly sensitive and specific enough for determining concentrations of SC in millicell system, and SC showed good transport in millicell system.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第1期57-60,共4页
China Journal of Chinese Materia Medica
基金
国家863计划(2003AA2Z347A)
关键词
槐果碱
LC—MS
CACO-2细胞
转运
sophocarpine
liquid chromatography-mass spectrometry
Caco-2 cells
transport
作者简介
[通讯作者]王广基，Tel：（025）83271544，E-mail：gjwang@cpu．edu．cn
