摘要
目的：应用分光光度法建立一种简便、准确、灵敏、稳定的重组α-半乳糖苷酶（AGA）活性检测方法。方法：利用紫外-可见分光光度计，根据产物生成量计算所测标本的酶活性。结果：该方法的批内变异系数为2．63％，批间变异系数为4．42％，均〈5％；在所检测该酶质量浓度范围（0．3～2．5）μg/ml）内线性关系良好；每组的平均回收率均在95％～105％之间。结论：与已经建立的测活方法相比较，该方法具有无需酶的标准品，结果可靠、误差小、稳定性好、操作简单，适合实验室及工业化生产应用的优点。
Objectlve：To establish a method that can be used for the precise determination of α-D-galactosidase activity. Method：Products produced by α-D-galactosidase were detected by means of spectrophotometric method, which could be used to evaluate α-D-galactosidase activity. Results：The results showed that the variability within batchs was 2.63% and the variability between batchs was 4.42%. These indicated that the method was reliable to could be used in α-D-galactosidase assay. Conclusion： The method has the merits of high precision, good reproducibility and easy operation, so it can be used for detecting α-D-galactosidase activity exactly.
出处
《军事医学科学院院刊》
CSCD
北大核心
2006年第6期559-561,共3页
Bulletin of the Academy of Military Medical Sciences
基金
国家重点基础研究发展规划("973"计划)(2002CB713804)
关键词
Α-半乳糖苷酶
活性测定
光谱分析
α-D-galactosidase
activity assay
spectroscopic analysis
作者简介
高红伟（1976-），女，山东省茌平县人，硕士。助理研究员。
通讯联系人，Tel：010-66931545.
