摘要
研究了环境激素壬基酚(4-NP)在雌鲫体内的对雌激素受体的表达和雌二醇水平的影响。雌鲫腹腔注射1、50、100 mg.kg-1的4-NP或1 mg.kg-1的E2,分别在24 h和48 h提取肝脏RNA用RT-PCR查看α型雌激素受体(ER-α)和CYP1A的表达情况和提取血清用荧光免疫法测定E2浓度。结果表明:1 mg.kg-1E2处理组的ER表达在24 h和48 h有显著性增加(P<0.01)。1 mg.kg-14-NP处理组在24 h ER表达与对照组比较有极显著性增加(P<0.01),在48 h有显著性增加(P<0.05),E2浓度在24 h与对照组比较有显著性下降(P<0.05),但在48 h后有所回升;CYP1A表达在处理后24 h有显著上调(P<0.05),在48 h后有极显著上调(P<0.01)。50 mg.kg-14-NP处理组在24 h ER表达与对照组比较有极显著性增加(P<0.01),在48 h有显著性增加(P<0.05),E2浓度在24 h和48 h与对照组比较有极显著性下降(P<0.01);CYP1A表达在处理后24 h有显著上调(P<0.05),在48 h后有极显著上调(P<0.01)。100 mg.kg-14-NP处理组ER表达在24 h和48 h与对照组比较有极显著性增加(P<0.01),E2浓度在24 h与对照组比较有显著性下降(P<0.05),在48 h有极显著性下降(P<0.01);CYP1A表达有极显著上调(P<0.01)。随着4-NP注射量的增加,ER表达也逐渐增加,而E2浓度逐渐减少。ER表达上调效应:E2>100 mg.kg-14-NP>50 mg.kg-14-NP>1 mg.kg-14-NP,100 mg.kg-1的4-NP对ER表达的上调效果接近于1 mg.kg-1E2的效果。E2下降效应:100 mg.kg-14-NP>50 mg.kg-14-NP>1 mg.kg-14-NP。NP对CYP1A表达有上调作用,并且表达随注射浓度上升而增强(具剂量-依赖效应)。以上结果表明,4-NP具有弱雌激素样效应,并且能抑制鲫鱼血清中E2的水平并使ER表达上调,为4-NP在鲫鱼体内通过ER途径实现内分泌扰乱效应的可能性提供了支持。
The estrogen-like effects of 4-nonylphenol （4-NP） in vivo of female Carassius auratus were investigated in this paper. We gave intraperitoneal injection in female Carassius auratus with the dose of 1 mg · kg^-1, 50 mg · kg^-1, 100 mg · kg^-1 of 4-NP or 1 mg · kg^-1 of 17β-estradiol （E2） respectively. The expression of α type estrogen receptor （ER-α） and CYP1A in liver was evaluated by RT-PCR and the concentration of E2 in serum was measured by fluorescent immunoassay in 24 h and 48 h of injection. The results show that 1 mg · kg^-1 of E2 increased expression of ER significantly both in 24 h and 48 h （P 〈 0.01 ）. In 4-NP treatment group, the concentration of E2 was decreased and the expression of ER was increased with the increase of 4-NP＇ s level. In 1 mg · kg^-1 of 4-NP treatment group, there is a significant increase of expression of ER at 24 h （ P 〈 0.01 ） and 48 h （ P 〈 0.05 ）, the concentration of E2 was decreased at 24 h （ P 〈 0.05） yet returned slightly at 48 h, and CYP1A＇ s expression is up-regulated at 24 h （P 〈0. 05） and 48 h （P 〈0.01 ）. In 50 mg · kg^-1 of 4-NP treatment group, there is a significant increase of expression of ER at 24 h （ P 〈 0.01 ） and 48 h （ P 〈 0.05 ）, the concentration of E2 was decreased significantly both at 24 h and 48 h （ P 〈 0.01 ）, and CYP1A＇ s expression is up-regulated at 24 h （ P 〈 0.05 ） and 48 h （P 〈0.01 ）. In 100 mg · kg^-1 of 4-NP treatment group, there is a significant increase of expression of ER both at 24 h and 48 h （ P 〈 0.01 ）, the concentration of E2 was decreased significantly at 24 h （ P 〈 0.05） and 48 h （ P 〈 0.01 ）, and CYP1A＇ s expression is up-regulated （ P 〈 0.01 ）. Effect of increasing of ER＇s expression is E2 〉 100 mg · kg^-1 4-NP 〉50 mg · kg^-1 4-NP 〉 1 mg · kg^-1 4-NP; effect of decreasing of concentration of E2 is 100 mg · kg^-1 4-NP 〉50 mg · kg^-1 4-NP 〉1 mg · kg^-1 4- NP. The effect of 100 mg · kg^-1 of 4-NP on ER＇ s expression is close to that of 1 mg · kg^-1 of E2, this indicates that 4-NP has weak estrogen-like effects. And 4-NP can up-regulate the expression of CYP1A （dose-dependent）. That increase in ER＇s expression and decrease in E2 in 4-NP treatment group provide support of 4-NP＇ s endocrine disruption via ER oathwavs in Carassius auratus.
出处
《水产学报》
CAS
CSCD
北大核心
2007年第1期7-14,共8页
Journal of Fisheries of China
基金
农业部海洋与河口渔业重点开放实验室资助(开-2-04-05)
上海市重点学科建设项目资助(Y1101)
关键词
壬基酚
α型雌激素受体
内分泌扰乱
雌激素样效应
内分泌干扰物
4-nonylphenol （ 4-NP ）
α type estrogen receptor （ ER-α ）
endocrine disruption
estrogen-like effects
endocrine disruptors
作者简介
刘青（1981-），男，贵州安顺人，硕士研究生，主要从事鱼类环境内分泌干扰研究。Tel：021—65710525，E-mail：q-liu@stmail．shfu．edu．cn
通讯作者：魏华，Tel：021-65710525；E-mail：hwei@shfll．edu．cn
