摘要
研究了有机染料中，陆红与脱氧核糖核酸（DNA）的结合反应，选择了实验的最佳条件：pH5．0的B—R缓冲溶液2．5mL，1．0×10^-3mol／L中性红溶液0．6mL。反应20min后体系的吸光度很稳定，在λ=530nm处有最大吸收峰，并且随着DNA的加入，中性红的吸收峰显著下降。因此以中性红为标记物，根据其在波长530nm处吸收峰下降的程度。可用于定量测定DNA。测量DNA的线性范围为0-10μg／mL，相关系数为0．998，该方法具有较高的灵敏度和选择性，已用于合成试样分析。
The binding reaction of DNA with Neutral Red has been studied. The optimum experimental conditions have been confirmed. In the medium of pH 5.0, 0.60 mL 1.0× 10-3 mol/L neutral red solution, the system shows an absorption peak （530 nm）, and this peak decreases with the increasing DNA concentration. A novel method for the determination of DNA at 530 nm using neutral red as a labeling agent was developed. The linear range of the DNA is 0 -10μg/mL with the correlation coefficient of 0.998. The method is of high sensitivity, and has been used to determine DNA in synthetic samples with satisfactory results.
出处
《分析试验室》
CAS
CSCD
北大核心
2007年第1期62-64,共3页
Chinese Journal of Analysis Laboratory
基金
安徽省教育厅(2006jql139)项目资助
关键词
中性红
脱氧核糖核酸
分光光度法
Neutral red
Deoxyribonucleic acid
Spectrophotometry
作者简介
赵丹华（1978-），女，讲师。
